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【目的】观察大腹皮、番泻叶、草果、砂仁、白术、枳壳、枳实等7种中药及乙酸龙脑酯、辛弗林等2种中药活性物质对比格犬慢性实验模型胃肠动力的影响。【方法】应用6只比格犬制备慢性实验模型,在全部犬的胃窦、十二指肠、空肠、回肠和结肠浆膜植入应力传感器,安装胃瘘供中药汤剂灌服,安装颈外静脉置管供静脉给药。造模后,每天观察比格犬空腹状态下胃肠运动特点,连续7 d。造模后第7天开始,记录给药前空腹状态下胃肠运动,作为基础对照,到消化间期移行性复合运动(MMC)I相,依次记录大腹皮、番泻叶、草果、砂仁、白术、枳壳、枳实(均经胃瘘给予煎剂200 m L),生理盐水(给予等体积灌胃),乙酸龙脑酯(为枳实、枳壳的主要活性物质,静脉输注),辛弗林(为砂仁活性物质,静脉输注)作用下的胃肠运动。全部犬1 d只观察1种药物,每种药物重复观察2次。通过植入的应力传感器记录清醒犬胃肠运动,观察MMC周期、收缩波频率、收缩波总数、收缩波振幅、平均振幅、动力指数(MI)。【结果】术后1~6 d,比格犬胃肠动力低下,未出现MMC周期。至术后第7天,在空腹状态下消化间期,胃窦、十二指肠、空肠和回肠均出现典型MMC周期,而结肠出现不规则MMC周期。与给药前及生理盐水组比较,大腹皮、乙酸龙脑酯干预后胃窦、十二指肠、空肠、回肠和结肠收缩波数及MI均升高,番泻叶、草果干预后十二指肠、空肠、回肠和结肠收缩波数及MI升高,砂仁干预后胃窦、十二指肠收缩波数及MI升高(均P<0.05)。枳壳干预后空肠MI降低,枳实干预后空肠、结肠MI降低,辛弗林干预后胃窦收缩波数和胃窦、空肠MI降低(均P<0.05)。【结论】应用比格犬慢性实验模型可有效进行胃肠动力中药筛选。大腹皮、番泻叶、草果、砂仁和乙酸龙脑酯可增强胃肠运动;枳实、枳壳和辛弗林可抑制胃肠运动;白术则对胃肠运动无影响。
【Objective】 The purpose of this study was to observe the effects of two traditional Chinese medicines, such as Da Zu Pi, Sennae, Fructus Mume, Amomum villosum, Atractylodes macrocephala, Citrus aurantium and Citrus aurantium, on the chronic experimental model of Beagle dogs Effects of gastrointestinal motility. 【Methods】 Six beagle dogs were used to prepare chronic experimental model. All dogs were implanted with stress sensors in gastric antrum, duodenum, jejunum, ileum and colon serosa. External venous catheter for intravenous administration. After modeling, observe the beagle dogs daily gastrointestinal motility characteristics, for 7 days. Starting from the 7th day after the model was established, the gastrointestinal motility was recorded before fasting, as the basic control, to the I phase of interphase (MMC) phase I, followed by recording of the large belly skin, senna, Amomum villosum, Atractylodes macrocephala, Citrus aurantium and Fructus aurantii (both 200 m L by decoction of gastric fistula), normal saline (given equal volume intragastric administration), bornyl acetate (the main active substance of Citrus aurantium and Citrus aurantium, Infusion), synephrine (Amomum active substance, intravenous infusion) under the action of gastrointestinal motility. All dogs 1 d observed only 1 drug, repeated observation of each drug 2 times. The implanted stress sensor was used to record the gastrointestinal motility of awake dogs. The cycles of MMC, the frequency of contractile waves, the total number of contractile waves, the amplitudes of contractile waves, the mean amplitudes and the motor index (MI) were observed. 【Results】 The beagle dogs had low gastrointestinal motility at 1 to 6 days after operation, and MMC cycles did not occur. At 7 days after operation, typical MMC cycles were found in the digestive tract, gastric antrum, duodenum, jejunum and ileum in the fasting state, and irregular MMC cycles in the colon. Compared with pre-administration and saline control, the wave numbers and MI of gastric antrum, duodenum, jejunum, ileum and colon were significantly increased after the treatment of big-bellied skin and borneol ester acetate The wave number and MI of the contraction of the intestine, jejunum, ileum and colon increased, while the number of antrum and duodenum contractile wave and the MI of the amygdala after the intervention of amomum (both P <0.05). After the intervention of Fructus Aurantii, the jejunum MI was decreased. The jejunum and colon MI were decreased after the intervention of Fructus Aurantii Immaturus and the number of gastric antrum contraction and MI of gastric antrum and jejunum decreased after synephrine intervention (both P <0.05). 【Conclusion】 The application of chronic experimental model of beagle dogs can effectively screen gastrointestinal motility traditional Chinese medicine. Large belly skin, senna, grass, Amomum and borneol acetate can enhance gastrointestinal motility; citrus aurantium, Citrus aurantium and Synephrine can inhibit gastrointestinal motility; Atractylodes on gastrointestinal motility has no effect.