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目的:研究骨形成蛋白-2(BMP-2)对低氧状态下人肺动脉平滑肌细胞(Pu lmonary artery smooth musc le cells,PASMCs)的PTEN(Phosohatase and tensin homolog deleted on chromosom e 10)表达调节作用。方法:将培养的人PASMCSs分为对照组(1%的氧浓度,5%的CO2和94%N2条件下培养)和BMP-2组(另加入BMP-2),培养48 h后通过CyQUANT细胞增殖检测试剂盒测定PASMCs的增殖,定量RT-PCR方法检测PTEN基因表达,W estern b lot方法检测PTEN蛋白的表达。结果:BMP-2组与对照组比较细胞增殖明显降低(P<0.05);定量RT-PCR结果显示,与对照组比较,BMP-2组细胞培养4 h、8 h、24 h后PTEN相对表达量(2-△△ct)均明显升高(P<0.05);进一步的western b lot证实BMP-2组PTEN蛋白表达也明显升高。结论:BMP-2能抑制缺氧状态下的PASMCs的增殖,通过上调PTEN表达可能是其机制之一。
AIM: To investigate the regulatory effect of BMP-2 on the expression of PTEN (Phosphatase and tensin homolog deleted of chromosom e 10) in human pulmonary artery smooth muscle cells (PASMCs) under hypoxia. Methods: The cultured human PASMCSs were divided into control group (1% oxygen concentration, 5% CO2 and 94% N2) and BMP-2 group (BMP-2 added) Proliferation assay kit was used to detect the proliferation of PASMCs. The expression of PTEN gene was detected by quantitative RT-PCR. The expression of PTEN protein was detected by Western blot. Results: Compared with the control group, the cell proliferation of BMP-2 group was significantly lower than that of the control group (P <0.05). The results of quantitative RT-PCR showed that the expression of PTEN in BMP-2 group was higher than that of the control group (2-△△ ct) were significantly increased (P <0.05); further western b lot confirmed PTEN protein expression in BMP-2 group was significantly higher. Conclusion: BMP-2 can inhibit the proliferation of PASMCs under hypoxia, which may be one of the mechanisms by up-regulating the expression of PTEN.