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目的:建立HPLC法测定广西红腺忍冬中绿原酸含量。方法:采用Hypersil ODS-2柱(4.6mm×250mm,5μm),以甲醇-乙腈-0.05%磷酸为流动相,梯度洗脱,流速0.5mL·min-1,检测波长238nm,柱温30℃。结果:对不同产地、不同加工方法的23个样品进行了绿原酸含量测定,绿原酸进样量在0.2～1.0μg范围内呈良好线性关系(r=0.9999),平均回收率(n=6)为100.5%(RSD=2.8%)。结论:不同产地及不同加工方法的样品中绿原酸含量差别较大,各样品绿原酸含量均远高于中国药典要求。
Objective: To establish a HPLC method for the determination of chlorogenic acid in Lonicera rugosa in Guangxi. METHODS: Hypersil ODS-2 column (4.6 mm×250 mm, 5 μm) was used with methanol-acetonitrile-0.05% phosphoric acid as the mobile phase. The flow rate was 0.5 mL·min-1. The detection wavelength was 238 nm and the column temperature was 30°C. RESULTS: Chlorogenic acid content was determined in 23 samples from different production areas and different processing methods. The linearity of the chlorogenic acid injection was in the range of 0.2-1.0 μg (r=0.9999). The average recovery rate (n= 6) is 100.5% (RSD = 2.8%). Conclusion: The chlorogenic acid content in samples from different producing areas and different processing methods varies greatly. The chlorogenic acid content of each sample is much higher than that of the Chinese Pharmacopoeia.