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目的:通过测定清络通痹精简方、单味雷公藤、雷公藤配伍三七、雷公藤配伍地黄组合中雷公藤3种毒性成分雷公藤甲素、雷公藤红素、雷公藤内酯甲的溶出含量,从化学成分角度探索“异类相制”减毒的规律,为雷公藤的临床配伍减毒应用提供实验依据。方法:利用高度选择性和灵敏度的三重四级杆质谱(LC-MS/MS)同时对3种有效成分——雷公藤甲素、雷公藤红素和雷公藤内酯甲进行检测,比较单味雷公藤组、雷公藤配伍三七组、雷公藤配伍地黄组、清络通痹精简方组中3种有效成分的溶出变化。结果:雷公藤药材水提液中雷公藤甲素的含量最高,雷公藤内酯甲次之,雷公藤红素的含量最低。而当雷公藤药材用量相同的情况下,雷公藤中3种有效成分在不同配伍环境下的溶出趋势一致,溶出顺序为单味雷公藤组>雷公藤配伍地黄组>雷公藤配伍三七组>清络通痹精简方组。结论:在配伍组合中三七对雷公藤3种有效成分的溶出有显著地抑制作用,地黄的抑制作用较弱,而清络通痹精简方中雷公藤3种有效成分的溶出明显降低。该研究结果为雷公藤的临床“异类相制”配伍减毒理论提供了实验依据。
OBJECTIVE: To determine the dissolution of triptolide triptolide, triptolide and triptolide triptolide in the combination of Tripterygium wilfordii with Tripterygium wilfordii, Tripterygium wilfordii and Tripterygium wilfordii, Content, from the perspective of chemical composition to explore “Heterogeneous phase system ” the law of attenuation, for the clinical compatibility of Tripterygium provide experimental basis for compatibility. Methods: Tripterygium wilfordii, tripton and triptolide were tested simultaneously by LC-MS / MS with high selectivity and sensitivity. Vine group, Tripterygium compatibility Panax group, Tripterygium compatibility group Rehmannia, Qingluo Tongbi decoction group three active ingredients dissolution change. Results: The content of triptolide in the water extract of Tripterygium wilfordii was the highest, and the content of triptolide and triprolide was the lowest. When Tripterygium wilfordii was used in the same dosage, the dissolution of the three active ingredients in Tripterygium wilfordii was the same under different compatibility conditions. The order of dissolution was Tripterygium wilfordii> Tripterygium wilfordii> Rehmannia> Tripterygium compatibility> Qinglintongbi concise side group. CONCLUSION: Panax notoginseng has a significant inhibitory effect on the dissolution of three effective constituents of Tripterygium wilfordii, and the inhibitory effect of Rehmannia glutinosa is weak. However, the dissolution of the three effective constituents of Tripterygium wilfordii decreased significantly in the Qingluotongbi concoction. The results of this study provide the experimental basis for the clinical “heterogeneous phase system” compatibility attenuation theory of Tripterygium wilfordii.