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目的:研究芦荟多糖(AP)的抗瘤作用及其机制。方法:采用移植性S_(180)肿瘤小鼠(实体瘤)和H_(22)肝癌小鼠(腹水瘤),单独ip或iv AP,或与环磷酰胺(CTX)、阿霉素(ADM)、氟脲嘧啶(FU)联合用药。连续给药10天后,S_(180)肿瘤小鼠剥取瘤块,称重,减毒试验则于处死前采血,化验血象,计算脾指数和胸腺指数;H_(22)肝癌小鼠停药后观察生存时间,或于停药后24小时取血,放射免疫分析法测IL-2和TNF含量。结果:AP 25mg/kg·d、50mg/kg·d两个剂量单独给药时,能显著降低S_(180)肉瘤移植小鼠瘤块重量,显著延长H_(22)肝癌移植小鼠的生存时间;与三种抗肿瘤药物合用时,均有不同程度的增效作用,且可减轻化疗药物的副作用;可显著提高荷瘤鼠血清中IL-2、TNF含量。结论:AP对移植性S_(180)肿瘤小鼠和H_(22)肝癌小鼠具有抗肿瘤作用及化疗增效和减毒的双重作用,其机理可能是诱生IL-2、TNF及提高机体免疫力。
Objective: To study the anti-tumor effect of aloe polysaccharide (AP) and its mechanism. METHODS: Transplanted S_(180) tumor mice (solid tumors) and H_(22) liver cancer mice (ascites tumors), ip or iv AP alone, or with cyclophosphamide (CTX), adriamycin (ADM) Combination of fluorouracil (FU). Ten days after continuous administration, tumors of S 180 mice were dissected and weighed. Before attenuating the test, blood was taken before sacrifice and blood was counted. Spleen index and thymus index were calculated; H_(22) liver cancer mice after drug withdrawal The survival time was observed, or blood was taken 24 hours after discontinuation of the drug. The levels of IL-2 and TNF were measured by radioimmunoassay. Results: When AP 25 mg/kg·d and 50 mg/kg·d were administered alone, the weight of tumor blocks transplanted in S 180 mice was significantly decreased, and the survival time of H 22 transplanted mice was significantly prolonged. When combined with three antitumor drugs, they all have different degrees of synergism and can reduce the side effects of chemotherapy drugs; it can significantly increase the serum levels of IL-2 and TNF in tumor-bearing mice. CONCLUSION: AP has anti-tumor effect and chemotherapy synergism and attenuation in grafted S 180 tumor and H 22 hepatocarcinoma mice. The mechanism may be induced IL-2, TNF, and increased body weight. Immunity.