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从东北地区20个市(县)玉米主产区采集玉米纹枯病标样,分离纯化得到茄丝核菌173株,对其进行菌丝融合群鉴定,共鉴定出AG1-IA、AG-5、AG4-HGⅡ等融合群以及双核菌。AG1-IA为东北地区的优势融合群,占分离菌株总数的75.57%;其次是AG-5融合群,占分离总数的18.86%;AG4-HGⅡ和双核菌株分别占1.14%和2.29%。随机选取不同地区的玉米纹枯病菌菌株64株(AG1-IA、AG5、AG4-HGⅡ、双核菌株),采用菌饼作为接种体,通过温室苗期接种鉴定,结果表明,同一融合群的菌株致病力存在明显分化现象。属于AG1-IA的菌株对3个玉米品种的平均致病力为21.88～80.21;AG-5的菌株也存在致病力分化现象,菌株对3个玉米品种的平均致病力为17.71～48.96。
A total of 173 strains of Rhizoctonia solani were isolated and purified from 20 maize main producing areas in Northeast China. The mycelial fusion population was identified and AG1-IA and AG-5 were identified , AG4-HG Ⅱ and other fusion groups and the dual-core bacteria. AG1-IA was the dominant fusion group in Northeast China, accounting for 75.57% of the total isolates. AG-5 fusion group was the second one, accounting for 18.86% of the total. AG4-HGⅡ and binuclear strain accounted for 1.14% and 2.29% respectively. Sixty-four strains of Rhizoctonia solani (AG1-IA, AG5, AG4-HGⅡ and binuclear strain) were randomly selected from different regions. The bacterial strains were used as inoculants and identified by inoculation in greenhouse. The results showed that the isolates of the same fusion group Pathogenicity obvious differentiation phenomenon. The average virulence of strains belonging to AG1-IA to three maize cultivars ranged from 21.88 to 80.21. Pathogenic differentiation of AG-5 strains was also observed. The average virulence of the strains to the three maize cultivars ranged from 17.71 to 48.96.