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目的 研究乳腺癌发生和发展相关基因群的表达及其初步功能。方法 用40 96种人类基因多聚酶链反应(PCR)产物制成BioDoor40 96型表达谱芯片,分离纯化正常乳腺组织和乳腺癌组织mRNA ,制备表达谱探针,用ScanArray3 0 0 0荧光扫描仪扫描芯片荧光信号图像,利用计算机分析正常乳腺组织和乳腺癌组织之间差异表达的基因。结果 在40 96种基因中,正常乳腺组织和乳腺癌组织之间差异表达的基因有619条(15 .11% )。生物信息学分析显示,这些差异表达的基因可能与乳腺癌的发生和发展密切相关。结论 乳腺癌的发生、发展中存在多基因表达调控的改变,对于相关基因群的研究有助于认识肿瘤发病机制,并为乳腺癌个体化治疗提供依据
Objective To study the expression and primary function of genes related to the occurrence and development of breast cancer. Methods 40 96 human gene polymerase chain reaction (PCR) products were used to make BioDoor40 96 expression microarray. The mRNA of normal breast tissue and breast cancer tissue was isolated and purified. The expression profiling probe was prepared. ScanArray 300 000 Fluorescent signal images were analyzed using computer for differentially expressed genes between normal breast tissue and breast cancer tissues. Results Of the 40 96 genes, 619 genes (15.11%) were differentially expressed between normal breast tissues and breast cancer tissues. Bioinformatics analysis showed that these differentially expressed genes may be closely related to the occurrence and development of breast cancer. Conclusion The occurrence and development of breast cancer have the regulation of multi-gene expression. The study of related gene groups is helpful to understand the pathogenesis of breast cancer and provide the basis for individualized treatment of breast cancer