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为了研究高频纳秒脉冲电场(ns PEFs)杀伤肿瘤细胞的机理,结合笛卡尔传输网格(CTL)和动态电穿孔等效电路模型对1个典型的2维多细胞系统在ns PEFs作用下的穿孔特性进行了仿真。利用Pspice仿真软件建立了该多细胞系统电路模型,并分析了其穿孔数量和穿孔半径变化特性。首先施加了10个场强、频率和脉宽分别为5k V/cm、1 MHz和500 ns的高频纳秒脉冲到该多细胞系统模型,结果表明随脉冲个数增加穿孔区域分布变化不大,而穿孔半径分布有明显的累积效应,细胞外膜和细胞核膜上部分孔的半径逐渐增大。同时比较了电场强度为3、5和10 k V/cm时该系统中穿孔数量和最大孔径随脉冲个数增加的变化情况。结果显示场强增加时穿孔数量明显增加,特别是场强从3 k V/cm提高到10 k V/cm时,系统整个细胞核膜从未穿孔到穿孔数量增加到2.658 4×105个。另外,穿孔半径仍然存在累积效应。仿真结果说明适当参数的高频ns PEFs能使细胞核膜发生穿孔,并起到扩大细胞部分穿孔半径的作用,可为后续实验研究选择高频纳秒脉冲参数提供依据。
In order to study the mechanism of tumor cells killing by ns PEFs, a typical 2-dimensional multicellular system was treated with ns PEFs in combination with Cartesian transport grid (CTL) and dynamic electroporation equivalent circuit model The perforation characteristics were simulated. The multi-cell system circuit model was established by Pspice simulation software, and the characteristics of perforation and perforation radius were analyzed. First, ten high-frequency nanosecond pulses with field strength, frequency and pulse width of 5k V / cm, 1 MHz and 500 ns were respectively applied to the multi-cell system model. The results showed that the distribution of perforation changed little with pulse number , While the perforation radius distribution has a significant cumulative effect, the outer membrane and the nuclear membrane on the part of the pore radius gradually increased. At the same time, the variation of the number of perforations and the maximum aperture with the increase of the number of pulses in the system at electric field intensities of 3, 5 and 10 kV / cm were compared. The results showed that the number of perforations increased obviously when the field strength increased. Especially when the field strength increased from 3 k V / cm to 10 k V / cm, the number of perforation from the non-perforation to the whole nuclear membrane increased to 2.658 4 × 105. In addition, there is still a cumulative effect of the perforation radius. The simulation results show that the appropriate parameters of high frequency ns PEFs can make the nuclear membrane perforation and play a role in expanding the cell part of the perforation radius, which can provide the basis for the subsequent experimental study choose high-frequency nanosecond pulse parameters.