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以产生ａ－淀粉酶的解淀粉芽孢杆菌（Ｂａｃｉｌｌｕｓａｍｙｌｏｌｉｑｕｅｆａｃｉｅｎｓ）ＷＤ－２１和不能利用淀粉的产生肌苷的枯草芽孢杆菌（ＢａｃｌｌｌｕｓＳｕｂｔｉｌｌｓ）ＨＷ－９（ａｄｅ，ＮＰ－，８－ＡＧ＇）为亲株，在分别以０．７５和０．５ｍｇ／ｍｌ的溶菌酶浓度作用下形成原生质体，在电镜观察下，其形成率为９９％。通过优化融合条件，即３５℃在ＰＥＧ－１０００溶液中融合，融合率达到３．１１×１０－６。得到的融合子在选择培养基上传代多次，淘汰掉不稳定的融合子，得到稳定的融合子Ｆ１６和Ｆ１２９具有产生ａ－淀粉酶的能力，在以淀粉为碳源的发酵培养基中摇瓶发酵试验，Ｆ１６菌株产生肌苷的能力接近６ｇ／Ｌ．
Bacillus amyloliquefaciens WD-21, which produces a-amylase, and Bacillus subtilis HW-9 (ade, NP-, 8-AG ’ Protoplasts were formed at concentrations of lysozyme of 0.75 and 0.5 mg / ml, respectively. The formation rate was 99% under the electron microscope. The fusion rate was 3.11 × 10-6 by optimizing the fusion conditions, ie, 35 ° C in PEG-1000 solution. The resulting fusion sub-passage on the selection medium several times, out of unstable fusions, to obtain a stable fusion F16 and F129 have the ability to produce a-amylase, starch in the fermentation medium as a carbon source In the flask fermentation test, the ability of the F16 strain to produce inosine approached 6 g / L.