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目的 研究IL—2与DTIC、DDP不同联合方案对B16黑色素瘤的细胞毒作用,为临床合理应用提供依据。方法 采用MTT法检测各实验组的光密度(OD值),计算杀伤率。结果 同时加IL—2组及先加IL—2,3小时后加DTIC组对B16抑制率降低,与单药DTIC比较有显著差异(t=3.05,P<0.05;t=5.51,P<0.01)。同时给DDP和IL—2组或先加IL—2,3小时后再加DDP组对B16抑制率影响不大;与单药DDP比较没有显著差异(t=0.43,P>0.05;t=0.49,P>0.05)。先加DTIC,3小时后再加IL—2组,与单药DTIC对B16的杀伤率比有所下降,但没有统计学显著差异(t=1.65,P>0.05)。先加DDP,3小时后再加IL—2组与单药DDP对B16的细胞毒作用相比,抑制率有显著提高(t=3.99,P<0.05)。结论 IL—2能提高DDP的细胞毒作用,且应在先使用化疗药物的基础上给药。
Objective To study the cytotoxic effect of different combinations of IL-2, DTIC and DDP on B16 melanoma, and provide evidence for its clinical application. Methods The optical density (OD) of each experimental group was measured by MTT method to calculate the killing rate. Results Compared with DTIC alone, IL-2 plus IL-2 and DTIC plus DTIC decreased the B16 inhibitory rate (t = 3.05, P <0.05; t = 5.51, P <0.01 ). At the same time, DDP and IL-2 group or IL-2 and 3-hour plus DDP group had little effect on the inhibition rate of B16. There was no significant difference with single-drug DDP (t = 0.43, P> 0.05; , P> 0.05). DTIC was added first 3 hours later plus IL-2 group, and the killing rate of single-agent DTIC to B16 was decreased, but there was no statistically significant difference (t = 1.65, P> 0.05). After adding DDP for 3 hours, the inhibitory rate was significantly increased (t = 3.99, P <0.05) compared with the cytotoxicity of B16 alone and IL-2 alone. Conclusion IL-2 can increase the cytotoxicity of DDP, and should be based on the use of chemotherapy drugs.