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目的 :比较单纯三价铁盐 (Fe)〔包括 Fe Cl3、Fe NH4 (SO4 ) 2 和 Fe(NO3) 3〕与三价铁盐次氮基三乙酸复合物 (Fe- NTA)诱导 DNA损伤的能力。方法 :通过 DNA结合溴乙锭后的荧光损失和DNA琼脂糖凝胶电泳的迁移率来测定 DNA的损伤程度。结果 :在过氧化氢和抗坏血酸存在的条件下 ,铁盐能够诱发小牛胸腺 DNA损伤 ,DNA损伤的程度与铁盐和过氧化氢呈剂量依赖关系 ,单纯铁盐 (Fe)所造成的 DNA损害程度远远低于 Fe- NTA。谷胱甘肽对于 Fe- NTA诱发的 DNA损伤具有保护作用。结论 :这一发现有助于我们理解 Fe- NTA诱发肾脏肿瘤的机理 ,有助于防治Fe- NTA的致癌作用。
OBJECTIVE: To compare the effects of simple ferric salts (Fe) [including Fe Cl3, Fe NH4 (SO4) 2 and Fe (NO3) 3] and ferricyanide nitrilotriacetic acid complex (Fe- NTA) ability. Methods: The degree of DNA damage was determined by the fluorescence loss after DNA bound to ethidium bromide and the mobility of the DNA agarose gel electrophoresis. RESULTS: Iron salts induced DNA damage in calf thymus in the presence of hydrogen peroxide and ascorbic acid. The extent of DNA damage was related to the dose-dependent relationship between iron and hydrogen peroxide and DNA damage caused by Fe alone The extent is far below that of Fe-NTA. Glutathione has a protective effect on Fe-NTA-induced DNA damage. Conclusion: This finding helps us to understand the mechanism of Fe-NTA-induced renal tumors and help prevent and control the carcinogenic effect of Fe-NTA.