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目的:检测用不同浓度梯度的克拉霉素干预后,离体鼻息肉组织中环氧合酶-2(COX-2)及核因子κB(NF-κB)的表达状况,探讨其在鼻黏膜炎症机制中的作用。方法:将鼻息肉组织块分别与克拉霉素(0、10-6、10-5及10-4mol/L)于体外共同培养1 d,应用Western blot和荧光实时定量PCR技术检测COX-2和NF-κB亚基的表达水平,观察克拉霉素干预后COX-2和NF-κB表达的变化规律。结果:对照组(0 mol/L克拉霉素组)鼻息肉组织中COX-2、NF-κBp50和NF-κBp65的蛋白质和核酸表达水平最高;随着克拉霉素干预剂量的增加,COX-2、NF-κBp50和NF-κBp65的表达水平呈剂量依赖性下降。相关分析表明,同一组鼻息肉组织中,COX-2核酸表达量分别与NF-κBp50、NF-κBp65呈直线正相关。结论:COX-2异常表达参与了鼻-鼻窦黏膜慢性炎症反应,克拉霉素可能通过阻断NF-κB通路来下调COX-2的合成,发挥其抗炎作用。
Objective: To detect the expression of cyclooxygenase-2 (COX-2) and nuclear factor κB (NF-κB) in nasal polyps after intervention with different concentrations of clarithromycin, Mechanism of the role. Methods: Nasal polyps were co-cultured with clarithromycin (0, 10-6, 10-5 and 10-4 mol / L) for 1 day respectively. Western blot and real-time quantitative PCR were used to detect the expression of COX-2 and NF-κB subunit expression levels observed changes in the expression of COX-2 and NF-κB after clarithromycin intervention. RESULTS: The protein and nucleic acid expressions of COX-2, NF-κBp50 and NF-κBp65 in the nasal polyps of 0 mol / L clarithromycin group were the highest. With the increase of the dose of clarithromycin, COX-2 , The expression of NF-κBp50 and NF-κBp65 decreased in a dose-dependent manner. Correlation analysis showed that in the same group of nasal polyps, COX-2 nucleic acid expression was positively correlated with NF-κBp50 and NF-κBp65 respectively. CONCLUSION: Aberrant expression of COX-2 is involved in chronic inflammatory reaction of nasal-sinus mucosa. Clarithromycin may down-regulate the synthesis of COX-2 by blocking NF-κB pathway and exert its anti-inflammatory effects.