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目的:研究新型磁性纳米脂质复合物对肝癌细胞和肝细胞的作用。方法:将肝癌细胞(Hep-G2)和肝细胞(L-02)根据加入的不同浓度新型纳米脂质复合物,各自分为空白对照组、不同浓度含铁脂质体组、空脂质体组,用MTT法观察各组细胞毒性,筛选出适合的浓度和时间;用普鲁士蓝染色、电镜检测观察各组细胞吞噬情况;用原子分光光度计观察各组细胞内铁含量;用细胞磁共振观察新型纳米脂质复合物体外显影效果。结果:新型磁性纳米脂质复合物含铁浓度在0.238μg/ml以上时,24小时有较强的细胞毒性,对肝癌细胞和肝细胞的抑制率均超过40%,故以下实验选择含铁浓度在0.238μg/ml以下;肝癌细胞和肝细胞与各浓度新型磁性纳米脂质复合物共同培养,24小时普鲁士蓝染色最高阳性率分别为5.5%和1.25%,24小时细胞内铁含量最高值分别为0.675pg/cell和0.460pg/cell;24小时电镜观察肝癌细胞和肝细胞空白对照组均未见颗粒样物质,各含铁脂质体组和空脂质体组均可见颗粒样物质;24小时肝癌细胞和肝细胞磁共振感兴趣区均未见明显信号表达。结论:新型磁性纳米脂质复合物含铁浓度在0.238μg/ml以下时,24小时细胞毒性较小;肝癌细胞对新型磁性纳米脂质复合物的吞噬作用稍高于肝细胞对其的吞噬;进入细胞内的新型磁性纳米脂质复合物含铁量低,体外显影效果不佳。
Objective: To study the effect of a novel magnetic nano-lipid complex on hepatoma cells and hepatocytes. Methods: Hepatoma cells (Hep-G2) and hepatocytes (L-02) were divided into blank control group, different concentrations of iron-containing liposome group, empty liposomes The cytotoxicity of each group was observed by MTT method, and the suitable concentration and time were screened. Prussian blue staining and electron microscopy were used to observe the phagocytosis of each group. The intracellular iron content was observed by atomic spectrophotometer. To observe the in vitro imaging effect of the novel nanolipid complex. Results: The new magnetic nano-lipid complex iron concentration above 0.238μg / ml, 24 hours have strong cytotoxicity, liver cancer cells and liver cells were more than 40% inhibition rate, so the following experiments select the iron concentration The results showed that the highest positive rate of Prussian blue staining was 5.5% and 1.25% at 24 hours, and the highest intracellular iron content at 24 hours Which were 0.675pg / cell and 0.460pg / cell respectively. No particle-like material was observed in 24 hours electron microscope observation of hepatocellular carcinoma cells and blank control group of hepatocytes. Particulate substances were observed in each of iron-containing liposome group and empty liposome group. No obvious signal expression was observed in the liver cancer cells and the regions of interest of the liver cells. CONCLUSION: The cytotoxicity of the novel magnetic nano-lipid complex at a concentration of 0.238μg / ml or less is less than that at 24 hours. The phagocytosis of hepatoma cells to the novel magnetic nano-lipid complex is slightly higher than that of the hepatoma cells. Into the cells of the new magnetic nano-lipid complex with low iron content, poor imaging in vitro.