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以超级杂交水稻“两优培九”为试验材料,运用蛋白质双向电泳技术研究了水稻生育后期不同氮素水平下(正常供氮水平的1/2,20 mg.L-1;正常供氮水平,40 mg.L-1;正常供氮水平的2倍,80 mg.L-1)叶片和籽粒蛋白质组水平的变化,并鉴定分析了其差异蛋白质点(共鉴定出16个叶片蛋白质、9个弱势粒蛋白质、4个强势粒蛋白质)的生物功能.结果表明:生育后期氮素是通过影响与光合有关的酶的活化、CO2的活化及光系统单位和电子传递链构成来影响和调节植物的光合作用;氮素可促进弱势粒中与能量合成和生长相关酶的表达;高氮水平不利于强势粒淀粉的合成,但充足的氮素对水稻物质累积及代谢具有重要作用.因此,在生育后期合理运用氮肥对提高水稻剑叶光合性能、增强源的功能、延缓功能性早衰及强化籽粒灌浆具有积极作用.
Using super hybrid rice “Liangyoupeijiu” as test material, the protein two-dimensional electrophoresis was used to study the effects of different nitrogen levels (1 / 2,20 mg.L-1 for normal nitrogen supply level, normal nitrogen supply level, 40 mg.L-1; 2-fold normal nitrogen supply, 80 mg.L-1) and identification of differential protein spots (16 leaf proteins were identified, 9 Weak grain protein and 4 strong grain protein). The results showed that: Nitrogen affects and regulates plant through influencing photosynthesis-related enzyme activation, CO2 activation and photosystem unit and electron transfer chain formation in later growth period Photosynthesis; Nitrogen promoted the expression of enzymes involved in energy synthesis and growth in inferior grains; high levels of nitrogen were not conducive to the synthesis of strong grain starch, but adequate nitrogen had an important role in the accumulation and metabolism of rice, The rational use of nitrogen fertilizer at the later stage has a positive effect on enhancing photosynthetic performance, enhancing source function, retarding functional premature aging and enhancing grain filling in rice flag leaf.