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本文研究了成人每周服用25mg乙胺嘧啶后,血浆中的药物浓度在体外对人骨髓细胞中二氢叶酸还原酶的抑制作用。二氢叶酸还原酶受药物抑制后将影响5,10-甲烯四氢叶酸的生成,从而影响脱氧尿嘧啶核苷酸(dU)转变成胸腺嘧啶脱氧核苷酸的甲基化过程。作者应用dU抑制试验测定dU转变成胸苷酸的甲基化作用,由此推测药物对二氢叶酸还原酶的抑制作用。从具有各种血液学紊乱的62例患者身
This study investigated the inhibitory effect of drug concentrations in plasma on dihydrofolate reductase in human bone marrow cells in vitro after 25 mg pyrimethamine was taken weekly in adults. Dihydrofolate reductase is inhibited by drugs will affect the 5,10-methotrexate tetrahydrofolate generation, thus affecting the deoxyuracil nucleotide (dU) into thymidine DNA methylation process. The authors used the dU inhibition assay to determine the methylation of dU into thymidylate, suggesting that the drug inhibits dihydrofolate reductase. From 62 patients with various hematological disorders