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为明确对大豆胞囊线虫病具有不同抗性的小黑豆品种的抗性机制,利用石蜡切片技术对线虫侵染不同抗性大豆品种根系的组织病理学差异进行了研究。结果表明:在侵染初期,抗感品种侵入根内的线虫数量没有明显差异,抗病品种形成合胞体的时间要早于感病品种辽豆15,哈尔滨小黑豆较早地在中柱内形成了合胞体,抗侵入能力较弱。随着线虫的侵染,抗病品种逐渐形成过敏性坏死反应,阻止线虫发育使根内形成非常少量的雌虫,其中灰皮支黑豆根内几乎没有雌虫形成,抗发育能力最强。感病品种辽豆15根内没有发生过敏性坏死反应形成了大量雌虫。在寄主维管束中,侵染后期中柱内形成的合胞体会使感病品种辽豆15木质部外移,根内导管数量减少,不利于水分运输的安全性。而3个抗病品种小黑豆的木质部组织结构没有发生病变,利于大豆的正常生长。
In order to clarify the resistance mechanism of small black soybean cultivars with different resistance to soybean cyst nematode, the histopathological differences of the roots infected by nematodes with different resistant cultivars were studied using paraffin section technique. The results showed that in the initial stage of infection, there was no significant difference in the numbers of nematodes invaded by resistant varieties, and the time for the formation of syncytia in susceptible cultivars was earlier than that in Liao bean 15 and Harbin black beans earlier in the middle column The syncytia, anti-invasive ability is weak. With the infection of nematodes, the resistant varieties gradually formed anaphylactic necrosis reaction, which prevented the development of nematodes to form a very small number of females in the roots. Few female pests were formed in the roots of the petiole, and the strongest resistance to development was found. Few susceptible varieties of Liao bean did not occur within 15 reactions to form a large number of females. In the host vascular bundle, the syncytia formed in the middle column during the late stage of infection will make the susceptible variety Liao bean 15 xylem migrate, and the number of the root canal is reduced, which is not conducive to the safety of water transport. The disease resistance of xylem in three resistant varieties of black beans did not change, which would benefit the normal growth of soybean.