基因枪转化技术在水稻的遗传转化上已被广泛地应用并获得显著的成效。与原生质体转化法相比较,基因枪法具有不受或少受基因型限制的优点且提高了转化效率,但对籼稻,仍有不少问题需要解决,转化系统尚须进一步完善。因此有必要对影响转化频率的因素进行深入的研究。我们在以barnase基因对籼稻的遗传转化以诱发工程雄性不育的研究中,特别注 The technology of gene gun transformation has been widely used in the genetic transformation of rice and achieved remarkable results. Compared with the protoplast transformation method, the gene gun method has the advantages of being less or less limited by the genotype and improving the transformation efficiency. However, for the indica rice, many problems still need to be solved and the transformation system needs to be further improved. Therefore, it is necessary to conduct in-depth research on the factors that affect the frequency of transformation. In our study of the genetic transformation of barnase to indica rice to induce engineered male sterility, we noted