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用人工合成的人胰岛素原类似物基因大肠杆菌表达产物免疫 BALB/ c小鼠制备免疫脾细胞 ,与小鼠骨髓瘤 SP2 / 0细胞系融合 ,建立了 7株能分泌抗胰岛素单克隆抗体 (Mc Ab)的杂交瘤细胞系。间接 EL ISA和免疫印迹试验结果表明 ,该单克隆抗体对人、牛及猪胰岛素具有特异性
BALB / c mice were immunized with the expressed product of synthetic human proinsulin analogue Escherichia coli to prepare immunized splenocytes and fused with mouse myeloma SP2 / 0 cell line to establish seven monoclonal antibodies that secreted anti-insulin monoclonal antibody (Mc Ab) hybridoma cell line. Indirect EL ISA and Western blotting results showed that the monoclonal antibody has specificity for human, bovine and porcine insulin