论文部分内容阅读
以‘丰香’(Toyonoka)和‘土特拉’(Tutela)草莓品种为试材,利用石蜡切片技术研究了在自毒物质对羟基苯甲酸胁迫下,尖孢镰刀菌草莓专化型(Fusarium oxysporum f.sp.fragariae)侵染根系引起的组织结构变化。结果表明:对羟基苯甲酸胁迫下,尖孢镰刀菌菌丝在‘丰香’和‘土特拉’根系中的侵染速率明显加快,对根系表皮、皮层、中柱薄壁细胞及导管壁结构的破坏程度也明显加重,且对‘丰香’的破坏程度明显重于‘土特拉’。对羟基苯甲酸胁迫显著提高了‘丰香’和‘土特拉’根系组织含菌丝导管的比例,分别为22.5%和15.8%;但‘土特拉’根系含胶状物导管的比例为26.3%,明显高于‘丰香’(16.3%)。在对羟基苯甲酸胁迫下,‘土特拉’根系组织产生胶状物比‘丰香’早。‘土特拉’单独接菌处理根系内含有侵填体导管的比例为1.6%,明显高于‘丰香’单独接菌处理和对羟基苯甲酸胁迫下‘土特拉’接菌处理(1.0%和1.2%)。对羟基苯甲酸胁迫下接种尖孢镰刀菌可明显加重草莓根系组织受损程度,且‘丰香’枯萎病发生较重。
Using Toyonoka and Tutela strawberry varieties as test materials, we studied the effects of Fusarium oxysporum fusarium oxysporum f.sp.fragariae) infiltration root structure changes. The results showed that under the stress of p-hydroxybenzoic acid, the infection rate of Fusarium oxysporum mycelium in ’Fengxiang’ and ’Putra’ roots was significantly accelerated. The root epidermis, cortex, central column parenchyma and catheter wall The extent of damage to the structure also significantly increased, and the damage to the abundance of incense significantly higher than the ’native’. The p-hydroxybenzoic acid stress significantly increased the proportion of mycelial catheters in ’Fengxiang’ and ’Mottra’ root tissues, which were 22.5% and 15.8%, respectively; however, the proportion of ’Toutra’ roots containing jelly catheters was 26.3%, significantly higher than ’Fengxiang’ (16.3%). Under the stress of p-hydroxybenzoic acid, the ’Tetra’ root system produces a gum that is earlier than the ’bland’. The proportion of tundra-containing catheter in root system treated with “phytase” alone was 1.6%, which was significantly higher than that of ’Fengtong’ alone and 1.0tuet treatment with p-hydroxybenzoic acid % And 1.2%). Inoculation of Fusarium oxysporum under the stress of p-hydroxybenzoic acid could significantly increase the damage of strawberry root tissue, and the occurrence of ’Fung Hong’ wilt occurred seriously.