本实验表明通常使用0.02%EDTA或0.25%Trypsin在常规条件下消化培养的细胞不会造成微绒毛的明显损伤,但要适当控制消化时间。消化时间过长能导致微绒毛的显著改变。经过EDTA处理细胞后必须用Hanks液清洗干净,否则残留于培养液中的EDTA对细胞可产生不良影响。用0.02%EDTA与0.25%Trypsin以1∶1等量混合制成的消化液对细胞的消化作用较好,对细胞膜微绒毛无损伤作用。 This experiment shows that cells usually digested with 0.02% EDTA or 0.25% Trypsin under normal conditions do not cause significant damage to microvilli, but control digestion time appropriately. Excessive digestion can result in significant changes in microvilli. After EDTA treatment, the cells must be washed with Hanks solution, otherwise EDTA remaining in the culture solution may have adverse effects on the cells. The digestion solution made by mixing 0.02% EDTA and 0.25% Trypsin in 1: 1 equal amount had better digestion effect on the cells and no damage on the cell membrane microvilli.