论文部分内容阅读
目的研究巨噬细胞移动抑制因子(MIF)在胃癌组织中的表达及临床意义,并探讨其机制。方法应用免疫组化(SP法)和革兰染色检测80例胃癌组和50例对照组的Hp-L型感染,免疫组化(SP法)检测上述组织MIF蛋白表达。结果胃癌组MIF表达阳性率高于其对照组(P<0.05),且表达水平与浸润深度、淋巴结转移、临床分期、分化程度有关(P<0.05),与性别、年龄无关(P>0.05);革兰染色、免疫组化两种方法检测Hp-L型的结果具有一致性(P<0.05),胃癌组与对照组的Hp-L型阳性率相比具有统计学意义(71.25%vs 14.00%,P<0.05)。胃癌组Hp-L型感染阳性率仅与淋巴结转移有关(P<0.05),Hp-L型阳性组的MIF表达阳性率高于Hp-L型阴性组(P<0.05),且Hp-L型阳性和MIF阳性呈正相关(r=0.598,P<0.05)。结论 MIF蛋白表达与胃癌的浸润转移密切相关,其机制可能与幽门螺杆菌L型(Hp-L型)感染有关。
Objective To study the expression and clinical significance of MIF in gastric cancer and to explore its mechanism. Methods Hp-L infection was detected by immunohistochemistry (SP method) and Gram stain in 80 gastric cancer patients and 50 control subjects. The expression of MIF protein was detected by immunohistochemistry (SP method). Results The positive rate of MIF in gastric cancer group was significantly higher than that in the control group (P <0.05), and the expression level was related to the depth of invasion, lymph node metastasis, clinical stage and differentiation (P <0.05) (P <0.05). The positive rates of Hp-L in gastric cancer group and control group were statistically significant (71.25% vs 14.00 %, P <0.05). The positive rate of Hp-L infection in gastric cancer group was only associated with lymph node metastasis (P <0.05). The positive rate of MIF expression in Hp-L positive group was higher than that in Hp-L negative group (P <0.05) There was a positive correlation between MIF positive and positive (r = 0.598, P <0.05). Conclusions The expression of MIF protein is closely related to the infiltration and metastasis of gastric cancer. The mechanism may be related to the infection of L type (Hp-L) of Helicobacter pylori.