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目的:了解Toll样受体2(TLR-2)在HBs Ag阳性孕妇胎盘组织中的表达和分布情况,探讨TLR-2经胎盘途径在乙肝病毒(HBV)宫内感染中的作用。方法:应用免疫组织化学ABC法检测TLR-2在HBs Ag阳性孕妇胎盘组织中的分布情况;采用荧光定量PCR法检测新生儿HBV DNA浓度。结果:17例HBs Ag阳性但新生儿未发生HBV宫内感染的孕妇中,7例胎盘组织TLR-2阳性,阳性率为41.18%;13例HBs Ag阳性而新生儿发生HBV宫内感染的孕妇中,11例胎盘组织TLR-2阳性,阳性率为84.62%,两者比较差异有统计学意义(P<0.05)。结论:胎盘组织TLR-2表达与HBV宫内感染有一定关系,胎盘屏障中TLR-2表达升高是发生HBV宫内感染的因素之一,但其发生机制有待进一步探讨。
Objective: To investigate the expression and distribution of Toll-like receptor 2 (TLR-2) in placenta of HBsAg-positive pregnant women and to explore the role of TLR-2 in the intrauterine infection of hepatitis B virus (HBV). Methods: Immunohistochemical ABC method was used to detect the distribution of TLR-2 in the placenta of HBsAg-positive pregnant women. Fluorescent quantitative PCR was used to detect the neonatal HBV DNA concentration. Results: Among 17 pregnant women with positive HBsAg but no neonatal HBV intrauterine infection, the positive rate of TLR-2 in 7 cases of placenta was 41.18%. Thirteen pregnant women with positive HBsAg and neonatal HBV intrauterine infection Among the 11 cases, the positive rate of TLR-2 in placenta tissue was 84.62%, the difference was statistically significant (P <0.05). CONCLUSION: The expression of TLR-2 in placenta is related to HBV intrauterine infection. The elevated expression of TLR-2 in placental barrier is one of the factors that cause HBV intrauterine infection, but its mechanism remains to be further explored.