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目的 研究直流电对肿瘤细胞膜通透性的影响及化疗增敏作用。方法 用直流电(6.0V,3.8mA,4.0C)作用于培养的ECA—109人食管癌细胞,用荧光示踪技术观察对ECA—109细胞膜通透性的影响。结果 荧光显微镜下可见LY(0.05％Luicifer Yellow mw457.2),能明显进入ECA—109人食管癌细胞内。但RD(Rhodamin Dextran mw:9000)则不能透入,将1/ 10~7细胞数破碎后,用分光光度计测其荧光强度,实验组为1.765±0.902,对照组为0.385±0.213(P<0.05)。这种现象在4小时以后逐渐消失。直流电也可以提高阿霉素(ADM,MW:579.99)进入细胞内的量。显微分光光度计分析,直流电加ADM组荧光强度为32.52±18.29,而对照组为8.05±4.09(P<0.05)。体外实验显示6.0V,3.8mA,2.0C作用于ECA—109细胞,其增殖抑率为14.0％,单纯ADM为11.2％(0.5μg/ml),直流电2.0C+ADM(0.5μg+/ml)为62.0％(P<0.05),其相互作用指数(CI)为0.67。结论 直流电能增强ECA—109人食管癌细胞膜通透性,体外实验条件下对阿霉素有增敏作用。
Objective To study the effect of direct current on the permeability of tumor cell membrane and the sensitization effect of chemotherapy. Methods DCA (6.0V, 3.8mA, 4.0C) was applied to cultured ECA-109 human esophageal cancer cells and the effect of the fluorescent tracer technique on the membrane permeability of ECA-109 cells was observed. Results Fluorescence microscopy showed that LY (0.05% Luicifer Yellow mw457.2) could obviously enter ECA-109 human esophageal cancer cells. However, RD (Rhodamin Dextran mw:9000) did not penetrate, and the number of cells was broken at 1/10~7. The fluorescence intensity was measured with a spectrophotometer. The experimental group was 1.765±0.902, and the control group was 0.385±0.213 (P< 0.05). This phenomenon gradually disappeared after 4 hours. Direct current can also increase the amount of adriamycin (ADM, MW: 579.99) that enters the cell. Microscopic spectrophotometer analysis, direct current plus ADM group fluorescence intensity of 32.52 ± 18.29, while the control group was 8.05 ± 4.09 (P <0.05). In vitro experiments showed that 6.0 V, 3.8 mA, and 2.0 C acted on ECA-109 cells, the proliferation inhibition rate was 14.0%, simple ADM was 11.2% (0.5 μg/ml), and direct current 2.0C+ADM (0.5 μg+/ml) was 62.0% (P<0.05), and its interaction index (CI) was 0.67. Conclusion DC power enhanced the membrane permeability of ECA-109 human esophageal cancer cells and sensitized to adriamycin under in vitro conditions.