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本实验研究了不同来源膳食蛋白质及其摄取水平对大鼠锌吸收、分布、潴留、排泄及生物利用率的影响。结果表明：发芽大豆在各项指标上均优于普通大豆（Ｐ＜０．０１）；粪锌及粪￣（６５）Ｚｎ排出随膳食蛋白质水平的升高呈明显下降趋势，低蛋白组（５％）最高；表现￣（６５）Ｚｎ吸收率，表现Ｚｎ吸收率及表现Ｚｎ潴留率皆随膳食蛋白质水平增加而增加。５％蛋白质水平组明显低于蛋白质≥１０％各组（Ｐ＜０．０１），而１０％、１５％、２０％三组数值相接近，无显著性意义（Ｐ＞０．０５），提示低蛋白降低锌吸收与潴留，增加内源锌排泄；而１０％蛋白质，１５ｍｇ／ｋｇ锌为蛋白质和锌较适宜的膳食含量。在方法学上比较观察了￣（６５）Ｚｎ示踪法和总锌法。结果显示：大鼠肝、肾、胫骨、脑、睾丸各脏器Ｚｎ及￣（６５）Ｚｎ潴留存在明显正相关关系（Ｐ＜０．０５），且表现￣（６５）Ｚｎ吸收率与表现Ｚｎ吸收率、表现Ｚｎ潴留率呈高度正相关Ｐ（＜０．０５），示踪法特异性强，灵敏度高，无内源锌干扰，建议广泛使用，替代总锌法。
The present study investigated the effects of different sources of dietary protein and its uptake on zinc absorption, distribution, retention, excretion and bioavailability in rats. The results showed that germinated soybean was better than common soybean in all the indexes (P <0.01). The excretion of Zn in feces and feces (65) decreased with the increase of dietary protein, %) Was the highest; Zn uptake, Zn uptake, and Zn retention were both increased with dietary protein levels. The levels of 5% protein were significantly lower than those of ≥10% protein (P <0.01), while the values of 10%, 15% and 20% were similar, with no significant difference (P> 0.05) Low protein decreased zinc absorption and retention, increased endogenous zinc excretion; and 10% protein, 15mg / kg zinc for protein and zinc more appropriate dietary content. The methodological comparison of ~ (65) Zn tracer and total zinc method was observed. The results showed that there was a significant positive correlation between Zn and Zn (65) Zn retention in liver, kidney, tibia, brain and testis (P <0.05), and the Zn absorption rate and Zn Absorption rate, the performance of the retention rate of Zn was highly positive correlation P (<0.05), Tracer method specificity, high sensitivity, no endogenous zinc interference, it is recommended that the widespread use of alternative total zinc method.