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目的和方法 :应用高效液相色谱法 (HPLC)测定了正常健康山羊内毒素 (ET)处理后 ,肝线粒体膜 (MiM)中磷脂酰肌醇 (PtdIns) ,磷脂酰丝氨酸 (PtdSer) ,磷脂酰乙醇胺 (PtdEtn)及磷脂酰胆碱 (PtdCho)的变化 ,同时用DPH探针标记红细胞膜 (ECM)和肝MiM制剂 ,采用分子荧光偏振技术测定了膜流动性的改变。结果 :ET处理组在处理后 5hPtdIns,PtdSer,PtdEtn ,PtdCho的含量明显低于对照组 (P <0 0 1) ,ECM和MiM的荧光各向异性 (A)显著高于对照组 (P <0 0 1)。结论 :ET可诱导膜磷脂含量的改变并对膜流动性有明显的影响
PURPOSE AND METHODS: The levels of PtdIns, PtdSer, phosphatidylserine (PtdSer) in the liver mitochondrial membrane (MiM) were determined by high performance liquid chromatography (HPLC) Ethanolamine (PtdEtn) and phosphatidylcholine (PtdCho). Meanwhile, DPH-labeled ECM and liver MiM preparations were used to measure the change of membrane fluidity by molecular fluorescence polarization. Results: The contents of PtdIns, PtdSer, PtdEtn and PtdCho in ET group were significantly lower than those in control group (P <0.01), and the fluorescence anisotropy (A) in ECM and MiM group was significantly higher than that of control group 0 1). Conclusion: ET can induce the change of membrane phospholipid content and have obvious influence on membrane fluidity