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［目的］观察核苷酸类似物拉米夫定联合泛昔洛韦体内抗鸭乙型肝炎病毒（ＤＨＢ）的作用。方法采用重庆麻鸭乙型肝炎动物模型，用拉米夫定联合泛普洛韦口服治疗４周，停药观察 １周，检测用药前后血清中的ＤＨＢＶ ＤＮＡ、ＤＨＢｓＡｇ及血清转氨酶（ＡＬＴ、ＡＳＴ）、肝组织ＨＥ染色病理。并以单用拉米夫定、泛昔洛韦、阿昔洛韦作对照。结果拉米夫定联会泛昔洛韦用药后能使血清中ＤＨＢＶ ＤＮＡ含量总体水平显著降低（Ｐ＜０．０１），停药１周后ＤＨＢＶＤＮＡ含量较用药 ４周时 ＤＨＢＶ ＤＮＡ含量回升现象不明显。用药前后血清 ＤＨＢｓＡｇ的吸光度值（４９０ ｎｍ）的变化与ＤＮＡ含量改变相似；此外，肝脏病理检查及治疗４周、停药１周后血清转氨酶检查未发现联合用药对鸭肝组织有明显的毒性损害。结论拉米夫定联合泛普洛韦连续用药４周在鸭体内有抗鸭乙型肝炎病毒的作用，且停药后ＤＨＢＶ ＤＮＡ无明显“反跳”， 二者用药有协同作用。
[Objective] To observe the effect of nucleotide analogue Lamivudine combined with Famciclovir against Duck Hepatitis B virus (DHB) in vivo. Methods The hepatitis B animal model of Chongqing ducklings was treated with lamivudine combined with aflatoxin for 4 weeks. The withdrawal time was 1 week. The serum DHPV DNA, DHBsAg and serum ALT (AST) , Liver tissue HE staining pathology. And lamivudine alone, famciclovir, acyclovir as a control. Results The overall level of DHBV DNA in serum after lamivudine conjugate famciclovir treatment was significantly decreased (P <0.01). The DHBV DNA content in serum was not significantly increased at 1 week after drug withdrawal. The change of absorbance value (490 nm) of serum DHBsAg before and after treatment was similar to that of DNA content. In addition, the serum aminotransferase test did not find obvious toxic damage to duck liver tissue after liver pathological examination and treatment for 4 weeks and 1 week . Conclusion Lamivudine combined with panoprofen for 4 weeks has the anti-duck hepatitis B virus resistance in ducklings, and DHBV DNA has no obvious anti-jumping after stopping the treatment. Both drugs have synergistic effect.