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为了分析研究光纤表面增强拉曼散射(SERS)探针的拉曼增强特性,采用简单廉价的化学方法制备Ag纳米颗粒修饰光纤SERS探针,采用罗丹明6G(R6G,Rhodamine 6G)作为探针分子进行了不同浓度R6G溶液(10-7~10-9 M)的拉曼测试实验和浓度为10-6 M的R6G溶液的Time-course SERS Mapping实验,研究了该光纤SERS探针的拉曼增强特性,制备的光纤SERS探针样品检测R6G的极限浓度低至10-9 M;在Time-course SERS Mapping实验中,分析验证了探针分子溶液蒸发过程对光纤SERS探针的拉曼增强特性的影响。研究表明,由于制备的光纤SERS探针对荧光噪声没有抑制作用,导致所测得探针分子的拉曼光谱受探针分子溶液状态、测试方式的极大影响。当溶液充足时(浸泡状态),所测光谱信号荧光噪声与有效拉曼光谱信号都比较大;在溶液干燥过程中,所测光谱信号荧光噪声与有效拉曼光谱信号都减少,但荧光噪声减小幅度远大于拉曼光谱信号。
In order to analyze Raman enhancement properties of SERS probes, Ag nanoparticles-modified SERS probes were prepared by a simple and inexpensive chemical method using R6G (Rhodamine 6G) as probe molecule The Raman test of R6G solution (10-7 ~ 10-9 M) and the Time-course SERS Mapping experiment of R6G solution of 10-6 M concentration were carried out. The Raman enhancement Characteristics, prepared SERS probe optical fiber samples to detect the limit concentration of R6G as low as 10-9 M; in the Time-course SERS Mapping experiment, the analysis confirmed that the probe molecule solution evaporation process SERS probe optical fiber Raman enhancement characteristics influences. The results show that the prepared SERS probe has no inhibitory effect on the fluorescence noise, resulting in the Raman spectrum of the probe molecule being greatly affected by the solution state of the probe molecule and the test mode. When the solution is sufficient (soaked state), the fluorescence signal of the measured fluorescence signal and the effective Raman spectrum signal are relatively large; in the process of solution drying, the fluorescence signal of the measured fluorescence signal and the effective Raman spectrum signal are reduced, but the fluorescence noise reduction Slightly larger than the Raman spectrum signal.