用外科方法从临床确诊的细粒棘球蚴病人肝脏取出包囊，以生发层为培养材料，采用ＲＰＭＩ１６４０、１９９、改良伊格水解乳蛋白培养基，用胶原蛋白包被和不包被两种培养瓶，对细粒棘球蚴生发层细胞进行初培养，并进行了对比观察。结果显示，以胶原蛋白作为支撑条件，应用改良伊格水解乳蛋白培养基培养生发层细胞的效果优于其他，并已传７代，原代培养时间需要２８～４５ｄ才能传代，３代以内细胞为多形态性。 Surgical methods were used to remove the cysts from the liver of patients with clinically diagnosed hydatidiform echinococcosis. The germinal layer was used as the culture material. RPMI1640 and 199 were used to improve the IgE hydrolyzed milk protein medium, coated with collagen and uncoated The culture flask was used to culture the germinal layer cells of Echinococcus granulosus and the contrast was observed. The results showed that using Collagen as a supportive condition, the effect of culturing germinal layer cells by using modified IgA hydrolyzed milk protein culture medium was better than others and it had been passed for 7 generations. Primary culture time required 28-45 days for passage, For more morphological.