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目的探讨毛橘红总黄酮在组织及细胞水平对酒精性肝损伤模型的影响。方法将雄性大鼠随机分为正常对照组、模型组、五子衍宗丸组(阳性药对照)及毛橘红总黄酮高、中、低剂量组,采用白酒灌胃造成酒精性肝损伤模型,造模同时进行药物治疗,28 d后取各组大鼠肝组织,常规HE染色,光镜观察。用乙醇体外诱导L-02肝细胞损伤,分别加入不同浓度的毛橘红总黄酮作用于L-02酒精性肝损伤细胞,检测培养液中丙氨酸氨基转移酶(ALT)和门冬氨酸氨基转移酶(AST)活性,MTT法检测肝细胞存活率。结果模型组肝呈典型酒精肝组织病变特征,毛橘红总黄酮各剂量组脂变程度、水肿程度以及炎性程度均有不同程度减轻;毛橘红总黄酮可显著降低L-02酒精性肝损伤细胞模型中的ALT、AST含量,增加细胞存活率。结论毛橘红总黄酮对大鼠酒精性肝病理损伤及L-02酒精性肝损伤细胞有一定保护作用。
Objective To investigate the effect of total flavonoids of Citrus grandis on alcohol-induced hepatic injury at the tissue and cellular level. Methods Male rats were randomly divided into normal control group, model group, Wuzi Yanzong Pill group (positive drug control) and high-, medium-, and low-dose groups of total flavonoids of C. oleifera. Alcohol-induced liver injury model was established by intragastric administration of white wine. At the same time, drug treatment was performed. After 28 days, liver tissues of rats in each group were taken for routine HE staining and observed under light microscope. Ethanol was used to induce L-02 hepatocyte injury in vitro. Different concentrations of total flavonoids were added to L-02 alcoholic hepatic injury cells. Alanine aminotransferase (ALT) and aspartic acid amino acids were detected in the culture medium. Transferase (AST) activity, MTT assay for hepatocyte viability. Results The livers of the model group were characterized by typical alcoholic liver lesions. The degree of lipidosis, degree of edema, and degree of inflammation of the total flavonoids were all reduced to varying degrees. The total flavonoids of the tangerine orange significantly reduced the L-02 alcoholic liver injury cells. The ALT and AST levels in the model increase cell survival. Conclusion Flavouring flavonoids of Trichosanthes acutus has protective effects on pathological injury of alcoholic liver and L-02 alcoholic hepatic injury in rats.