论文部分内容阅读
[目的]检测新疆维吾尔族宫颈癌及癌前病变患者人乳头状瘤病毒16型(HPV16)存在状态,探索其与维族宫颈病变进展关系。[方法]对HPV16阳性维族宫颈鳞癌(SCC)和宫颈上皮内瘤变(CIN)活检标本采用多重实时PCR检测HPV16 E2和E6拷贝数,通过E2/E6比值判断HPV16 DNA的存在状态。[结果]随着宫颈病变程度加重,游离型HPV16逐渐减少,并且随着宫颈病变级别的升高,HPV16整合比率(混合型+整合型)逐渐上升。在CINⅠ、CINⅡ/Ⅲ、SCC以及宫颈癌Ⅰ~Ⅳ期中,整合率(1-E2/E6)都呈上升趋势,但无统计学差异。[结论]HPV16 E2基因断裂可能是新疆维吾尔族妇女宫颈癌的致病因素之一,但尚不能认为整合率是评价维吾尔族宫颈癌前病变进展的参考指标之一。
[Objective] To detect the existence status of human papillomavirus type 16 (HPV16) in Uygur cervical cancer and precancerous lesions in Xinjiang Uygur Autonomous Region, and to explore its relationship with the progression of Uygur cervical lesions. [Methods] Multiplex real-time PCR was used to detect the copy number of HPV16 E2 and E6 in HPV16-positive Uighur cervical squamous cell carcinoma (SCC) and cervical intraepithelial neoplasia (CIN) biopsy specimens. The E2 / E6 ratio was used to determine the presence of HPV16 DNA. [Results] With the severity of cervical lesions, free HPV16 gradually decreased, and with the increase of cervical lesions, HPV16 integration ratio (mixed + integrated) gradually increased. In the CINⅠ, CINⅡ / Ⅲ, SCC and cervical cancer Ⅰ ~ Ⅳ stage, the integration rate (1-E2 / E6) showed an upward trend, but no statistical difference. [Conclusion] The breakage of HPV16 E2 gene may be one of the causative factors of cervical cancer in Uyghur women in Xinjiang, but it can not be considered that the integration rate is one of the reference indexes for evaluating the progression of cervical precancerous lesions in Uighur.