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以甲基丙烯酸为功能单体,戊唑醇(tebuconazole,TEB)为模板分子,乙二醇二甲基丙烯酸酯为交联剂,应用电化学聚合法制备了分子印迹电化学传感器(TEB-MIP/F-CNTs/GCE)。利用循环伏安法(cyclic voltammetry,CV)和交流阻抗法(electrochemical impedance spectroscopy,EIS)对新型传感器进行了表征,并用差分脉冲伏安法(differential pulse voltammetry,DPV)考察了新型传感器对戊唑醇的检测性能。结果表明:戊唑醇分子印迹电化学传感器具有一定特异性;传感器表观表面积比裸电极显著提高;新型传感器具有良好的印迹效果,相较于其他结构类似的三唑类化合物(如苯醚甲环唑),TEB-MIP/F-CNTs/GCE对戊唑醇表现出高效的识别能力,具有专一性和强选择性,且在C_(TEB)≤2.0×10~(-6)mol/L范围内传感器峰电流与C_(TEB)存在定量关系;数据模拟分析得到传感器的电学阻抗谱等效电路模型为R_1(CPE_1(R_2(CPE_2(R_3)))),计算各电极/传感器的等效电路中各元件参数,证明该模型能有效地模拟传感器检测戊唑醇的传感机理。该研究结果可为三唑类农药残留仿生免疫检测方法的建立提供技术支撑。
Molecularly imprinted electrochemical sensors (TEB-MIPs) were prepared by electrochemical polymerization using methacrylic acid as functional monomer, tebuconazole (TEB) as template and ethylene glycol dimethacrylate as crosslinker. / F-CNTs / GCE). The new sensor was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The effects of the new sensor on tebuconazole The detection performance. The results showed that tebuconazole molecular imprinted electrochemical sensor has certain specificity; the apparent surface area of sensor is significantly higher than that of bare electrode; the new sensor has good imprinting effect. Compared with other triazole compounds with similar structure (TEB) ≤2.0 × 10 ~ (-6) mol / L for TEB-MIP / F-CNTs / GCE for tebuconazole and its specificity and strong selectivity. L is the quantitative relationship between the peak current of the sensor and C_ (TEB); the equivalent circuit model of the electrical impedance spectrum obtained by the data simulation is R_1 (CPE_1 (R_2 (CPE_2 (R_3)))) It is proved that the model can effectively simulate the sensing mechanism of tebuconazole by the sensor. The results of this study can provide technical support for the establishment of biomimetic immunoassay for triazole pesticide residues.