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作者采用凝胶电泳ABC染色法,以P21单克隆抗体为探针,在凝胶上直接进行免疫酶联染色,对鼻咽癌、宫颈癌,慢性鼻咽炎、慢性宫颈炎及正常宫颈组织和胎鼻咽组织进行了ras癌基因的产物P21表达的研究。结果表明,鼻咽癌及宫颈癌中P21具有过量表达,其阳性率分别为78.9％和70％。6例慢性鼻咽炎与5例宫颈炎中各有1例阳性,正常宫颈和胎鼻咽组织均为阴性。结果显示,P21在鼻咽癌及宫颈癌中的表达明显增强。
The authors used ABC gel staining method to P21 monoclonal antibody as a probe on the gel directly on the enzyme-linked immunosorbent assay for nasopharyngeal carcinoma, cervical cancer, chronic nasopharyngitis, chronic cervicitis and normal cervical tissue and fetal Nasopharyngeal tissues were studied for the expression of the P21 gene, a product of the ras oncogene. The results showed that P21 was over-expressed in nasopharyngeal carcinoma and cervical carcinoma, the positive rates were 78.9% and 70% respectively. One case was positive in 6 cases of chronic nasopharyngitis and 5 cases of cervicitis. The normal cervical and fetal nasopharyngeal tissues were all negative. The results showed that P21 in NPC and cervical cancer significantly increased expression.