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以抗根肿病大白菜自交系‘YM-7’和感病自交系“冠291”及其杂交F1、F2代和24份国内外引进的大白菜种质资源为试材,采用人工接种和分子标记方法,研究了2对与抗根肿病基因Crr3紧密连锁分子标记的稳定性及24份新材料中Crr3基因的情况。结果表明:引物OPC11-2S在‘YM-7’中扩增出1条1.3kb片段,在“冠291”中扩增出1条1.0kb片段,在其F1代中同时扩增出1.3kb和1.0kb的片段,该分子标记在F2代中的鉴定结果与人工接种鉴定结果一致,利用引物OPC11-2S能准确标记出大白菜种质资源中Crr3基因,而且能够区分出Crr3基因的纯合性和杂合性,共选出2份含有杂合Crr3基因的新种质资源。
The Chinese cabbage germplasm resources introduced from Chinese cabbage and ’YM-7’ and susceptible inbred line ’Guan291’ and their F1 and F2 hybrids were used as test materials, The stability of two pairs of molecular markers tightly linked to the clubroot gene Crr3 and the situation of Crr3 gene in 24 new materials were studied by artificial inoculation and molecular marker methods. The results showed that a 1.0kb fragment was amplified from the primer OPC11-2S in ’YM-7’, and a 1.0kb fragment was amplified in the ’crown 291’ kb and 1.0kb fragments. The identification results of F2 and F2 were the same as those of artificial inoculation. The primer OPC11-2S could accurately identify the Crr3 gene in Chinese cabbage germplasm resources, and could distinguish the pure Crr3 gene 2 new hybrid germplasm resources containing heterozygous Crr3 gene were selected.