目的了解饲喂Ⅱ型胶原(typeⅡcollagen ,CⅡ)的大鼠能否在其肠相关淋巴组织和脾脏中 ,诱导产生CⅡ特异性T细胞及其分泌的细胞因子的类型。方法对口服CⅡ致耐大鼠进行特异和非特异性淋巴细胞增殖实验 ,并采用酶联免疫斑点法 (ELISPOT) ,对CⅡ特异性T细胞在单细胞水平进行细胞内细胞因子检测。结果CⅡ致耐大鼠的T细胞增殖能力下降 ,CⅡ特异性T细胞多数分泌TGF β,极少数分泌IL 4和IFN γ。结论多次口服小剂量CⅡ可在佐剂性关节炎(adjuvantarthritis ,AA)大鼠模型中 ,诱导产生分泌TGF β的CⅡ特异性T细胞(Th3细胞) ,抑制AA的进展。 Objective To investigate whether rats fed type Ⅱcollagen (CⅡ) can induce CⅡ-specific T cells and their secreted cytokines in their gut-associated lymphoid tissues and spleens. Methods Specific and non-specific lymphocyte proliferation was induced in C Ⅱ -induced rats. ELISPOT was used to detect intracellular cytokine levels of CⅡ-specific T cells at the single-cell level. Results The proliferation ability of T cells induced by CⅡ was decreased in rats. Most of CⅡ-specific T cells secreted TGFβ, and a few of them secreted IL-4 and IFNγ. Conclusions CⅡ-specific T cells secreting TGFβ can be induced by multiple oral doses of C Ⅱ in a rat model of adjuvant arthritis (AA), which inhibits the progression of AA.