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小麦(Triticum aestivum L.)品质相关性状是由多个基因控制的数量性状,发掘控制小麦品质相关性状的真实主效数量性状位点(quantitative trait loci,QTL)是利用分子手段进行品质改良的有效途径。本研究收集了已发表的控制小麦品质相关性状的585个QTL定位数据,利用元分析技术获得264个控制小麦品质相关性状一致性QTL(meta quantitative trait loci,MQTL),其中34个与吸水率(water absorption,ABS)相关,26个与面粉蛋白质含量(flour protein content,FPC)相关,84个与籽粒蛋白质含量(grain protein content,GPC)相关,52个与籽粒硬度(kernel hardness,KH)相关,39个与沉降值(sedimentation value,SV)相关,29个与湿面筋含量(wet gluten content,WGC)相关。基于Bio Mercator软件及标记映射技术,将来自不同遗传作图群体的小麦品质相关性状QTL整合到一张参考图谱上,建立控制小麦品质相关性状的QTL一致性图谱,进一步发现控制小麦品质相关性状的MQTL区段在染色体上呈非均匀性分布,且部分性状QTL集中成簇。控制小麦品质相关性状的真实QTL区段(或位点)的发现及其整合图谱的构建,为小麦品质相关性状基因的发掘、候选基因的确定以及分子标记辅助选育提供了参考资料。
Quality-related traits in wheat (Triticum aestivum L.) are quantitative traits controlled by multiple genes. Discovering the true trait loci (QTLs) controlling the trait related to wheat quality is the effective use of molecular means for quality improvement way. In this study, 585 QTLs for controlling wheat quality-related traits were collected and 264 meta-trait loci (MQTLs) were obtained by meta-analysis. Among them, 34 were associated with water absorption ( 26 were related to flour protein content (FPC), 84 were related to grain protein content (GPC), 52 were related to kernel hardness (KH) 39 were related to sedimentation value (SV), and 29 were related to wet gluten content (WGC). Based on Bio-Mercator software and marker-mapping technology, the QTLs for wheat quality-related traits from different genetic mapping populations were integrated into a reference map to establish a QTL mapping map for controlling wheat quality-related traits. It was further found that controlling QTLs for wheat quality-related traits The MQTL segments were heterogeneous in chromosomes, and some traits of QTLs clustered. The discovery of the true QTL segment (or locus) controlling the wheat quality-related traits and the construction of its integrated map provide references for the exploration of genes related to wheat quality, determination of candidate genes and molecular marker-assisted breeding.