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目的探讨不同剂量的矽尘和不同染尘时间对大鼠血清和支气管肺泡灌洗液(BALF)中克拉拉细胞蛋白(Clara cell protein,CC16)和肺泡表面活性蛋白D(surfactant protein D,SP-D)表达水平的影响,探讨矽肺的早期诊断新指标。方法制作矽尘致大鼠肺纤维化模型,矽尘剂量和染尘时间各组不同,测定大鼠BALF中羟脯氨酸(HYP)含量、BALF和血清中CC16及SP-D含量以及肺组织中CC16表达的免疫组化染色分析。结果 BALF中CC16的含量在染尘后第3天(49.65 ng/mg)、第7天(47.10 ng/mg)、第14天(46.15 ng/mg)、第21天(41.63 ng/mg)、第28天(38.02 ng/mg),均较对照组(分别为61.27 ng/mg,60.67 ng/mg,61.20 ng/mg,60.61 ng/mg,56.76 ng/mg)显著降低(P<0.05),并出现下降趋势;血清中CC16的含量在第7天(9.8 ng/ml)、第14天(9.36 ng/ml)、第21天(9.0 ng/ml)、第28天(8.78 ng/ml)也较对照组(11.04 ng/ml,10.84 ng/ml,10.88 ng/ml,10.96 ng/ml)显著降低(P<0.05)。大鼠血清中SP-D的含量较对照组显著下降(P<0.05),BALF中SP-D含量随染尘时间变化出现抛物线趋势。肺组织CC16免疫组化显示Clara细胞损伤严重,CC16表达降低。只有50 mg/ml染尘组BALF的HYP高于对照组(P<0.05)。结论在矽尘引起肺组织病变过程中CC16和SP-D具有肺组织特异性,可能作为矽肺的一个早期标志物。
Objective To investigate the effects of different dosages of silica dust and different dusting time on the expression of Clara cell protein (CC16) and surfactant protein D (SP-1) in rat serum and bronchoalveolar lavage fluid (BALF) D) expression of the impact of early detection of silicosis new indicators. Methods Silica dust induced rat model of pulmonary fibrosis, silica dust dose and dusting time were different in different groups, measuring the contents of hydroxyproline (HYP) in BALF, BALF and serum contents of CC16 and SP-D and lung tissue In the expression of CC16 immunohistochemical staining. Results The level of CC16 in BALF was significantly higher in BALF than that in BALF at day 3 (49.65 ng / mg), day 7 (47.10 ng / mg), day 14 (46.15 ng / mg), day 21 On the 28th day (38.02 ng / mg), it was significantly lower than that of the control group (61.27 ng / mg, 60.67 ng / mg, 61.20 ng / mg, 60.61 ng / mg, 56.76 ng / And showed a downward trend. The content of CC16 in serum on day 7 (9.8 ng / ml), on day 14 (9.36 ng / ml), on day 21 (9.0 ng / ml) (P <0.05) compared with the control group (11.04 ng / ml, 10.84 ng / ml, 10.88 ng / ml, 10.96 ng / ml) The content of SP-D in serum decreased significantly (P <0.05). The SP-D content in BALF showed a parabolic tendency with the change of dusting time. Immunohistochemical staining of CC16 in lung tissue showed that Clara cells were severely damaged and CC16 expression was decreased. Only HYP in BALF of 50 mg / ml dyed group was higher than that in control group (P <0.05). Conclusion CC16 and SP-D have lung tissue specificity during the pathological changes of lung tissue induced by silica dust, which may be an early marker of silicosis.