论文部分内容阅读
目的:筛选胃癌(gastric cancer,GC)转移相关的差异表达微小RNA(micro RNA,miR NA),探讨miR NA-210与GC转移的可能机制.方法:应用mi RNA表达谱芯片筛选GC高转移细胞株RF48及低转移细胞株RF1的差异表达mi RNAs,RT-PCR检测mi RNA-210在7种GC细胞株中的表达情况,利用mi RWalk软件获得mi RNA-210的靶基因,通过David在线软件(包括GO分析及KEGG通路预测)分析mi R-210靶基因的可能生物学功能.结果:与RF1相比,mi RNA芯片在RF48细胞中共筛选出21个表达上调和15个表达下调的mi RNA,其中mi RNA-210在高转移细胞株RF48中的表达增高;RT-PCR结果显示m i R N A-210在高转移G C细胞株中表达增高(P<0.05);mi RWalk共获得155个mi RNA-210靶基因;GO分析及KEGG pathway分析得到miR NA-210靶基因功能,这些靶基因参与了肿瘤的发生、发展及转移.结论:利用mi RNA芯片技术获得了GC转移相关mi RNA表达谱;mi RNA-210的异常表达可能与GC的转移有关,为GC转移的早期诊断及发现新的治疗靶点奠定了基础.
OBJECTIVE: To screen differentially expressed microRNA (miR NA) involved in the metastasis of gastric cancer (GC) and to explore the possible mechanism of miR-210 and GC metastasis.Methods: Mi RNA expression profiling was used to screen GC highly metastatic cells The mi RNAs were differentially expressed in RF48 and low metastatic cell lines RF1. The expression of mi RNA-210 in seven GC cell lines was detected by RT-PCR. Mi RNA-210 target gene was obtained by mi RWalk software. (Including GO analysis and KEGG pathway prediction) were used to analyze the possible biological functions of mi R-210 target gene.Results: Compared with RF1, mi RNA microarray screened 21 miRNAs with up-regulated expression and 15 down-regulated expression in RF48 cells , Mi RNA-210 was highly expressed in high-metastatic cell line RF48; RT-PCR results showed that mi RN A-210 was highly expressed in highly metastatic GC cell lines (P <0.05) -210 target genes, GO analysis and KEGG pathway analysis of miR-210 target gene function, these target genes involved in the tumorigenesis, development and metastasis.Conclusion: Mi RNA chip miRNA expression profiles obtained by miRNA chip technology; Aberrant expression of miRNA-210 may be related to the metastasis of GC, which is GC Early diagnosis and the discovery of new therapeutic targets laid the foundation.