胰岛β细胞是糖尿病研究的中心环节之一。目前体外研究胰岛β细胞的模型系统有两类,一是从胰岛β细胞瘤组织克隆产生的β细胞株,二是从活体胰岛组织分离纯化获取胰岛β细胞。国内利用细胞株作为研究模型者较多,而β细胞的体外原代培养尚未见研究报道。从正常胰腺组织分离纯化取得的胰岛虽可反映其实际生理功能,但纯化后所得胰岛数量相对不足,而且胰岛内血流丰富,氧需求量大,分离过程中常发生缺血缺氧,特别是胰岛中央细胞(多数为β细胞)会因营养分配不均发生坏死。另外,分 Islet β-cell is one of the central aspects of diabetes research. At present, there are two types of model systems for studying islet β cells in vitro. One is the β cell line cloned from pancreatic β-cell tumor tissue and the other is pancreatic β-cell isolation and purification from the living pancreatic islet tissue. Domestic use of cell lines as a research model are more, and β-cell in vitro primary culture has not been reported. Isolation and purification of islets from normal pancreatic tissue may reflect the actual physiological function, but the amount of islets after purification is relatively insufficient, and the blood flow in the islets is abundant, the oxygen demand is large, and ischemia and hypoxia often occur during the isolation, especially islets Central cells (mostly beta cells) undergo necrosis due to unequal nutrient distribution. In addition, points