论文部分内容阅读
用超声法制备了内部包裹亚油酸铂，表面有抗人乳腺癌单克隆抗体ＭｅＡｂＧｐ－１Ｄ８的亚油酸铂靶向脂质体和亚油酸钠非靶向脂质体。研究了这些脂质体经腹部注射到荷瘤裸鼠之后的组织分布和抑瘤效果。实验结果表明，把向脂质体亚油酸钠在肿瘤组织的含量明显高于游离亚油酸铂组；在肾、肝、肺、脾、心脏等器官中，前者的含量比后者有所降低。分别在接种癌细胞后６天、１２天和２４天，按６ｍｇ／Ｋｇ的剂量分别注射ＰＢＳ，游离亚油酸铂，亚油酸铂脂质体和亚油酸铂靶向脂质体３次，在４０天观察结果。实验发现，无论在动物存活数、肿瘤发生率还是在肿瘤生长速度方面，亚油酸铂靶向脂质体的抑制能力都明显在优于游离亚油酸钠。用马来酰来亚胺自旋标记（ＭＳＬ）技术研究了亚油酸铂靶向脂质体与肿瘤细胞的相互作用以及它们对ＥＳＲ谱的影响，实验表明，马来酰亚胺自旋标记化合物（ＭＳＬ）标记癌细胞的ＥＳＲ谱是由两种波叠加而成。亚油酸铂的作用使乳腺细胞和Ｓ１８０实体癌细胞的Ｗ／Ｓ值降低，表明，亚油酸铂作用于膜蛋白引起膜蛋白构象变化，使马来酰亚胺自旋标记化合物（ＭＳＬ）运动受到限制加强。我们的实验提示，亚油酸铂抗癌机理可能是先与癌细胞细胞膜上的蛋白质发生作用，然后才进入细胞内与Ｄ?
The liposomal platinum linoleate and sodium linoleate non-targeted liposomes were prepared by ultrasonic method, which were coated with platinum linoleate and anti-human breast cancer monoclonal antibody MeAbGp-1D8. The tissue distribution and anti-tumor effect of these liposomes were investigated after they were injected into the tumor-bearing nude mice. The experimental results showed that the content of sodium linoleate liposome in the tumor tissue was significantly higher than that of free linoleate group; in the kidney, liver, lung, spleen, heart and other organs, the former than the latter reduce. Six days, 12 days and 24 days after the inoculation of cancer cells, PBS, free linoleic acid platinum, linoleic acid liposome and linoleic acid platinum were respectively injected into the liposomes at a dose of 6 mg / Kg for 3 times Observe the result at 40 days. The results showed that the inhibition of liposomal targeting of linoleic acid was significantly better than that of free linoleic acid, both in terms of the number of animals surviving, the incidence of tumors and the rate of tumor growth. The interaction of platinum linoleate targeting liposomes with tumor cells and their effect on ESR spectra were studied by using maleimide-imide spin labeling (MSL) technique. The experiments show that the maleimide spin labeling The ESR spectrum of compound (MSL) labeled cancer cells is a combination of two types of waves. The action of linoleic acid platinum reduced the W / S value of breast cells and S180 solid cancer cells, indicating that the action of platinum linoleate on the membrane protein confers a conformational change in the membrane protein and the maleimide spin-labeled compound (MSL) Exercise is limited to strengthen. Our experiments suggest that linoleic acid platinum anti-cancer mechanism may be the first with the cancer cell membrane protein on the role before it enters the cell and D?