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目的 测定对人不致病的鼠疫耶尔森菌布氏田鼠疫源地菌株 910 0 1的全基因组序列 ,并通过比较基因组学研究 ,探索鼠疫耶尔森菌致病性的遗传学机制和进化路线。方法 采用全基因组鸟枪法测定 910 0 1菌株的全基因组序列 ,利用比较基因组方法对 910 0 1与另外 2株鼠疫耶尔森菌(CO92和KIM)的全基因组进行比较研究。结果 910 0 1菌株的基因组包括 1条染色体和 4个质粒 (pPCP1、pCD1、pMT1和pCRY)。pPCP1质粒长度为 96 0 9bp,与参考菌株基本一致 ;pCD1质粒是编码Ⅲ型分泌系统的质粒 ,长度为70 15 9bp,编码情况与参考菌株基本相同 ,但重排导致了质粒的结构与参考菌株存在差异 ;pMT1质粒长度为10 6 6 4 2bp,保留了较多的原始质粒片段 ,毒力相关基因与参考菌株没有差异 ;pCRY质粒是本研究中新发现的质粒 ,在国内外研究中未曾报道 ,这一质粒长度为2 174 2bp ,具有独立复制能力 ,有一群编码Ⅳ型分泌系统的基因。 910 0 1菌株的染色体长度为4 5 95 0 6 5bp,有 4 0 37个编码序列 (CDS) ,其中 14 1个是假基因 ;染色体中存在着非常丰富的插入序列 ,由于存在IS序列介导的基因组重排 ,910 0 1菌株染色体的结构与参考菌株存在较大差异。通过比较基因组学分析 ,初步确定了910 0 1菌株甘油降解阳性、硝酸盐还原阴性、阿拉?
Objective To determine the whole genome sequence of non-pathogenic Yersinia pestis strain 910 0 1 and to explore the genetic mechanism and evolution of Yersinia pestis pathogenicity by comparing genomics research route. Methods Whole genome sequence of 910 0 1 strain was determined by genome-wide shotgun and the genomes of 910 0 1 and 2 strains of Yersinia pestis (CO92 and KIM) were compared by comparative genomic method. Results The genome of the 910 0 1 strain consisted of one chromosome and four plasmids (pPCP1, pCD1, pMT1 and pCRY). The plasmid pPCP1 was 96 0 9 bp in length, which was consistent with the reference strain. The pCD1 plasmid was a plasmid encoding type Ⅲ secretion system with a length of 70 15 9 bp. The coding sequence of pPCP1 was similar to that of the reference strain. However, rearrangement resulted in the structure of the plasmid and the reference strain There was a difference. The length of pMT1 plasmid was 10 6 6 4 2bp, more original plasmid fragments were retained, and the virulence-related genes did not differ from the reference strains. The pCRY plasmid was a newly discovered plasmid in this study and was not reported in domestic and foreign research This plasmid is 2 174 bp in length and has an independent replication capacity with a group of genes encoding the type IV secretion system. 910 0 1 strain had a chromosome length of 4595 0 6 5bp with 4 037 coding sequences (CDSs), of which 14 1 were pseudogenes; a very abundant insert exists in the chromosome and is present due to the IS sequence-mediated The genomic rearrangement of 910 0 1 strain showed a significant difference from the reference strain. By comparative genomics analysis, 910 0 1 strains were initially identified glycerol degradation positive, nitrate reduction negative, Allah?