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为了解木薯细胞壁酸性转化酶基因Me CWINV1胁迫诱导模式及调控机制,本研究利用PCR的方法,从木薯基因组中分离到一段Me CWINV1基因启动子序列(Gen Bank登录号:KC465190)。分析结果显示,该启动子长度865 bp,含有TATA box和CAAT box保守元件,以及MBS、HSE、AT1等多个与植物逆境胁迫相关的元件。通过构建GUS基因融合表达载体,对该启动子在烟草中的瞬时表达进行分析。结果表明,GUS基因主要在烟草叶脉中表达。研究结果为进一步研究该启动子的功能奠定了基础。
In order to understand the mechanism and regulation of Me CWINV1 stress induction in cassava cell wall, a promoter region of Me CWINV1 gene (GenBank accession number: KC465190) was isolated from cassava genome by PCR. The results showed that the promoter was 865 bp in length and contained many conserved TATA box and CAAT box elements as well as MBS, HSE, AT1 and other elements related to plant stress. The transient expression of this promoter in tobacco was analyzed by constructing GUS gene fusion expression vector. The results showed that GUS gene was mainly expressed in tobacco veins. The results laid the foundation for further study on the function of this promoter.