1082例乙型肝炎表面抗原阳性准确性分析

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目的为保证乙肝表面抗原(HBsAg)检测阳性结果报告的准确性,避免医疗纠纷。方法酶联免疫法(ELISA法)检测HBsAg,阳性结果用胶体金法(GICA法)复检。两者不符时分析原因,再次用ELISA法复检,最后确定结果。结果用ELISA法检测的1087例HBsAg阳性标本,用GICA法复检,其中6例HBsAg为阴性,分析原因,再次用ELISA法复检,确定5例为假阳性,而1082例为阳性。结论为保证HBsAg阳性检验结果的准确性,建议将ELISA法检测HBsAg阳性的标本用GICA法复检,两者不符时,分析原因,再次用ELISA法复检,最后确定结果。 Objective To ensure the accuracy of the reported positive results of HBsAg detection and avoid medical disputes. Methods HBsAg was detected by enzyme - linked immunosorbent assay (ELISA). The positive results were retested by GICA method. Analysis of the reasons when the two do not match, re-ELISA re-examination, and finally determine the results. Results A total of 1087 HBsAg positive samples were detected by ELISA. Six of them were negative for HBsAg. The reasons were analyzed again. ELISA was used again to confirm that 5 cases were false positive and 1082 cases were positive. Conclusion In order to ensure the accuracy of HBsAg positive test results, it is suggested that HBsAg positive samples should be rechecked with GICA method by ELISA. When they do not match with each other, the reasons are analyzed and the results are re-examined by ELISA.
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