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丙二醛(MDA)是生物分子氧化的典型羰基中间体,是脂质过氧化的典型终末产物.这类活性羰基中间体引起组织蛋白的老化与恶化及DNA损伤.牛磺酸在人体内具有非常广泛的生理功能,而γ-氨基丁酸(GABA)是神经系统的一种重要抑制性神经递质,因此二者是两种很重要的非蛋白氨基酸.研究了在生理条件下牛磺酸或GABA是否直接诱捕MDA从而保护组织蛋白不受伤害.将牛磺酸或GABA与MDA在生理条件下温浴48 h,经高效液相、质谱等分析手段分析,发现牛磺酸或GABA与MDA直接反应,通过HPLC分离,都生成两种主产物,一种是没有荧光的紫外吸收峰为274～278 nm的产物,一种是有荧光的产物,这种类似脂褐素(Ex.392～395 nm/Em.456～364 nm)的荧光产物是1,4-二氢吡啶衍生物.牛磺酸或GABA与MDA的反应表明它们在生理条件下具有清除活性羰基的功能,而这种毒性羰基与很多羰基紧张相关的疾病及衰老有关.
Malondialdehyde (MDA) is a typical carbonyl intermediate for biomolecule oxidation and is a typical terminal product of lipid peroxidation, which leads to the aging and deterioration of tissue proteins and DNA damage. Taurine in the human body Has a very wide range of physiological functions, and γ-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the nervous system, so they are two important non-protein amino acids.Studied under physiological conditions taurine Acid or GABA directly trap MDA to protect the tissue protein from injury.Taurine or GABA and MDA were incubated under physiological conditions for 48 h and analyzed by high performance liquid chromatography and mass spectrometry analysis and found that taurine or GABA and MDA Direct reaction, by HPLC separation, are produced two main products, one is no fluorescence of the UV absorption peak of 274 ~ 278 nm products, one is a fluorescent product, this is similar to lipofuscin (Ex. 395 nm / Em.456 ~ 364 nm) is a 1,4-dihydropyridine derivative.The reaction of taurine or GABA with MDA indicates that they have the function of scavenging the active carbonyl group under physiological conditions, and this toxicity Carbonyl and many carbonyl tension-related diseases and aging related .