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2007年冬季在江苏省兴化市和南京市一些日光温室大棚中发生了一种新的番茄病毒病,主要表现为植株矮缩,叶片上卷,叶缘黄化等,危害十分严重。为明确其病原,对田间表现典型症状的20份病样进行了分子检测,结果全部检测到粉虱传双生病毒;对其中12份进行序列同源性分析,发现其同源性超过98%,说明其极可能是同一个分离物;将该序列与GenBank进行BLAST分析,发现其与番茄黄化曲叶病毒的同源性最高,达99%。
In the winter of 2007, a new tomato virus disease occurred in some greenhousehouses in Xinghua City, Jiangsu Province and Nanjing City. The main diseases were tomato plant dwarfing, leaf rolling and leaf margin yellowing, which were very serious. In order to clarify the pathogen, 20 samples with typical symptoms in the field were detected by molecular analysis, all of them were detected as whitefly-transmitted geminiviruses. Sequence homology analysis of 12 of them showed that the homology was more than 98% Indicating that it is most likely the same isolate; BLAST analysis of the sequence with GenBank showed that it had the highest homology with Tovirus of Tomato (99%).