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目的分析大鼠ig宝儿康散后在血浆中的暴露成分,为其作用机制及药动学研究奠定基础。方法采用超高效液相色谱-高分辨飞行时间质谱联用技术(UPLC-Q-TOF/MS),分离给药后吸收入血成分。根据化合物的保留时间,相对分子质量及二级质谱特征碎片,结合文献报道,分析鉴定大鼠血浆中宝儿康散化学成分。结果大鼠ig宝儿康散1 h后,在血浆中分离鉴定了23个化合物,其中有5个化合物通过与对照品比对保留时间与质谱数据,确定了化学结构,分别为牡荆素、甘草素、橙皮素、甘草次酸、熊果酸。结论本方法可以快速分析宝儿康散大鼠ig给药后血浆暴露成分,对进一步探究其作用机制及其药动学奠定了基础。
OBJECTIVE: To analyze the exposure components of plasma in rats after ig Boer Kang Kang, and to lay a foundation for its mechanism of action and pharmacokinetics. Methods Ultra-high performance liquid chromatography-high resolution time of flight mass spectrometry (UPLC-Q-TOF / MS) was used to separate and infect blood components after administration. According to the compound retention time, relative molecular mass and second-order mass spectrometry fragments, combined with the literature reports, the chemical components of rat’s blood samples were identified. Results ig Baixie Kang after 1 h, isolated and identified in the plasma of 23 compounds, of which 5 compounds by comparison with the reference substance retention time and mass spectrometry data to determine the chemical structure, namely vitexin, Liquiritigenin, hesperetin, glycyrrhetinic acid, ursolic acid. Conclusion This method can rapidly analyze the plasma exposure components of Baonikangsan rats after ig administration, which lays the foundation for further exploring its mechanism of action and its pharmacokinetics.