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将勐旺谷、云南药用野生稻和燕麦等外源供体材料的DNA,通过花粉管通道法和孕穗期茎注射法导入水稻栽培品种西南175,获得了一批性状变异并稳定遗传的材料。变异类型包括:生育期、株型、穗型、稻瘟病抗性、谷粒蛋白质和氨基酸含量、酯酶和过氧化物酶同工酶等。这些变异,有的为供体特有性状,有的则是新性状。从482份导入后代中,获得各类变异材料17份,变异率3.5％;其中稻瘟病抗性增强的材料7份,抗性变异率1.45％。实验表明:通过花粉管通道法或者孕穗期茎注射法将外源DNA导入水稻,均可以引起受体后代的变异,转移供体性状,培育单性状转移和优良性状集中的新品种。
DNA from Mengwanggou, Yunnan medicinal wild rice and oat and other exogenous donor materials were introduced into rice cultivars southwest 175 by pollen tube channel method and booting induction at booting stage, and a number of materials with stable and inherited traits were obtained . Variation types include: growth period, plant type, panicle type, blast resistance, grain protein and amino acid content, esterase and peroxidase isozymes. These variations, some for the donor-specific traits, while others are new traits. From 482 imported progenies, 17 varieties of mutants were obtained, with a variation rate of 3.5%. Among them, 7 were resistant to rice blast and the rate of resistance mutation was 1.45%. Experiments show that introduction of exogenous DNA into rice through pollen tube pathway or stem-shoot injection at booting stage can induce the variation of the offspring of the recipient, transfer the donor traits, and cultivate new varieties with single trait transfer and excellent trait concentration.