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目的：研究外源性ｐ１６基因对胶质瘤细胞系ＳＨＧ－４４的抑制作用，探索胶质瘤基因治疗的新途径．方法：利用携带４９０ｂｐ的人ｐ１６全长ｃＤＮＡ真核表达载体，采用脂质体介导法将其导入人恶性胶质瘤细胞系ＳＨＧ－４４中，用Ｇ４１８筛选阳性克隆，以原位杂交，免疫组化方法检测ｐ１６基因在细胞转染前后的表达情况，应用流式细胞仪，电子显微镜，生长曲线测定等方法研究ｐ１６基因对胶质瘤细胞周期，形态，生长等特性的影响．并观察导入外源性ｐ１６基因的胶质瘤细胞对裸鼠致瘤能力的影响．结果：转染ｐ１６基因的ＳＨＧ－４４细胞内有外源性ｐ１６基因的表达，细胞周期明显变化，细胞从Ｇ１期到Ｓ期发生抑制，细胞有退行性改变，细胞增殖活性降低．结论：转染外源性ｐ１６基因可阻止胶质瘤细胞由Ｇ１期进入Ｓ期，并抑制肿瘤细胞增殖．
OBJECTIVE: To study the inhibitory effect of exogenous p16 gene on glioma cell line SHG-44 and explore a new approach for gene therapy of glioma. METHODS: Human p16 full-length cDNA carrying 490 bp was used to transfect it into human glioblastoma cell line SHG-44 by liposome-mediated method. Positive clones were screened by G418 and in situ hybridization was performed. The expression of p16 gene was detected by histochemical method before and after transfection. Flow cytometry, electron microscopy and growth curve were used to study the effects of p16 gene on cell cycle, morphology and growth of glioma. And observe the effect of glioma cells with exogenous p16 gene on the tumorigenicity of nude mice. RESULTS: The expression of exogenous p16 gene was detected in SHG-44 cells transfected with p16 gene. The cell cycle was changed obviously. The cells were inhibited from G1 phase to S phase. The cells had degenerative changes and the cell proliferation activity was decreased. Conclusion: The transfection of exogenous p16 gene can prevent Glioma cells from entering the S phase from G1 phase and inhibit the proliferation of tumor cells.