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本文观察了蛋白激酶C（PKC）抑制1－（5－异喹啉磺酰基）－2－甲基哌嗪（H－7）和槲皮素对血小板激活因子（PAF）体内作用的影响，结果发现：特异性PAF受体拮抗剂WEB2086可有效抑制内毒素（LPS）的致死毒性，进一步说明PAF是内毒素休克的重要介质；ivPAF可诱导小鼠腹腔毛细血管通透性增高（1．0μg／Kg）或死亡（25μg／Kg），PKC抑制剂H－7、槲皮素对二者都具有显著的抑制作用，且显著相关，提示H－7刊槲皮素抑制PAF毒性与保护内皮细胞有关；PAF在体内对小鼠的毒性和对微血管的损伤．可能与PKC有关。
This article investigates the effect of protein kinase C (PKC) on the in vivo effects of 1- (5-isoquinolinesulfonyl) -2-methylpiperazine (H-7) and quercetin on platelet activating factor (PAF) The results showed that: the specific PAF receptor antagonist WEB2086 can effectively inhibit the lethal toxicity of endotoxin (LPS), further demonstrating that PAF is an important mediator of endotoxic shock; ivPAF can induce increased peritoneal capillary permeability (1.0μg / Kg) or death (25μg / Kg), PKC inhibitor H-7 and quercetin had significant inhibitory effect on both of them, suggesting that the inhibition of PAF toxicity by H-7-quercetin is related to the protection of endothelial cells PAF toxicity to mice and microvascular damage in vivo. May be related to PKC.