论文部分内容阅读
目的:探讨大鼠急性酒精中毒合并颅脑外伤后AQP4在海马区表达的变化。方法:健康成年雄性SD大鼠96只,随机分为4组:假手术组(N组)、急性酒精中毒组(A组)、中度创伤性脑损伤组(T组)和急性酒精中毒合并中度创伤性脑损伤(AT组)。腹腔注射酒精(2.5g/kg),2h后以重物自由落体击打大鼠头部建立急性酒精中毒合并中度创伤性脑损伤(traumatic brain injury,TBI)动物模型。各组动物分别存活1、3、5、14天。免疫组化方法检测海马CA1区AQP4的表达。结果:AQP4阳性产物分布于胶质纤维和毛细血管壁,各实验组表达均高于N组。术后1天T组比AT组表达显著增高(P<0.01),术后3天AT组比T组表达增高(P<0.05),术后14天AT组比T组表达显著增高(P<0.01)。结论:大鼠急性酒精中毒合并颅脑外伤后晚期,海马CA1区AQP4表达增高,可能加重晚期继发性脑水肿,是急性酒精中毒合并颅脑外伤预后不良的原因之一。
Objective: To investigate the changes of AQP4 expression in hippocampus after acute alcoholism and traumatic brain injury in rats. Methods: Ninety-six healthy adult male Sprague-Dawley rats were randomly divided into 4 groups: sham operation group (N group), acute alcoholism group (A group), moderate traumatic brain injury group (T group) and acute alcoholism Moderate traumatic brain injury (AT group). The rats were injected intraperitoneally with alcohol (2.5g / kg). After 2 hours, the rats were hit by free weights to establish the animal model of acute alcoholism and traumatic brain injury (TBI). Each group of animals survived 1,3,5,14 days. Immunohistochemistry was used to detect the expression of AQP4 in hippocampal CA1 region. Results: The positive products of AQP4 distributed in the glial fibrils and capillary wall, the expression of each experimental group were higher than the N group. The expression of T in AT group was significantly higher than that in AT group on the 1st day after operation (P <0.01), the expression of AT group was higher than that of T group on the 3rd day after operation (P <0.05) 0.01). Conclusion: AQP4 in hippocampal CA1 region may be aggravated in rats with acute alcoholism complicated with traumatic brain injury, which may aggravate advanced secondary cerebral edema. It is one of the reasons for poor prognosis of acute alcohol poisoning combined with craniocerebral trauma.