目的：ＭＴＴ法观察三七总甙的体外细胞毒性与抗肝纤维化活性。方法：传代培养ＮＩＨ／３Ｔ３ 细胞，不同浓度接种细胞、不同血清浓度培养、不同ＭＴＴ用量等观察ＭＴＴ的转化Ａ５７０值，建立合适的试验条件。分别以无血清或含５％ 小牛血清的１６４０ 培养液稀释不同浓度的三七总甙或三七浸膏冻干粉，温育细胞１８ ｈ，测定ＭＴＴ转化Ａ５７０ 值，观察三七总甙及三七对ＮＩＨ／３Ｔ３ 细胞的毒性作用与对细胞增殖的影响。结果：细胞数量与ＭＴＴ Ａ５７０值明显正相关，在一定范围内血清温育浓度及ＭＴＴ用量与ＭＴＴ Ａ５７０值也呈明显正相关。三七总甙浓度＞ １．６ ｍ ｇ／ｍ ｌ时，对细胞有明显毒性作用，其ＩＤ５０ 约为０．４ ｍ ｇ／ｍ ｌ，对小牛血清刺激的细胞增殖有明显的抑制作用。与三七冻干粉相比，三七总甙对细胞的毒性作用与增殖抑制影响均较明显。结论：三七总甙能抑制成纤维细胞增殖，具有体外抗肝纤维化作用，可能是三七的抗肝纤维化的主要药效成分。 Objective: To observe the in vitro cytotoxicity and anti-hepatic fibrosis activity of Panax notoginseng by MTT assay. Methods: NIH/3T3 cells were subcultured, different concentrations of cells were inoculated, different concentrations of serum were cultured, different amounts of MTT were used to observe the AMT values ​​of MTT, and appropriate experimental conditions were established. Diluted different concentrations of P. notoginseng or Panax notoginseng lyophilized powder in serum-free or 1640 medium with 5% calf serum, and incubate the cells for 18 h. Determine the MTT-transformed A570 value, observe the Panax notoginseng total and The toxic effects of Panax notoginseng on NIH/3T3 cells and its effect on cell proliferation. Results: The number of cells was positively correlated with the MTT A570 value. There was also a positive correlation between serum concentrations of MTT and MTT and MTT A570 values ​​within a certain range. When Panax pseudoginseng has a total limulus concentration of >1.6 μg/ml, it has obvious toxic effects on cells, and its ID50 is about 0.4 μg/ml, which significantly inhibits the proliferation of cells stimulated by calf serum. Compared with Panax notoginseng freeze-dried powder, Panax notoginseng has obvious effects on cell toxicity and proliferation inhibition. Conclusion: Panax notoginseng can inhibit the proliferation of fibroblasts and has anti-fibrotic effect in vitro. It may be the main effective component of anti-fibrosis in Panax notoginseng.